Development of high resolution imaging for tumor boundary delineation using 7-tesla magnetic resonance imaging enhanced by high iron diamine immersion

使用高铁二胺浸没增强的 7 特斯拉磁共振成像开发用于肿瘤边界描绘的高分辨率成像

• 批准号: 24659283
• 负责人: MAESAWA Chihaya
• 金额: $ 2.25万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659283/
• 关键词: MRI; 病理; 高鉄ジアミン染色; EMR; ESD; 臨床病理; LST; 大腸癌; 臨床病理学

We developed a method for ex vivo imaging using 7-tesla high-resolution magnetic resonance imaging (MRI) combined with high-iron diamine (HID) immersion.All MR images were acquired with a 7-tesla scanner by spoiled gradient recalled acquisition using the steady state (SPGR) gradient-echo sequence. Colorectal specimens obtained from normal rats and model rats with colon cancer induced by dimethylhydrazine (DMH) were enhanced by immersion in colloidal iron or HID as contrast agents for goblet cell mucin, and subsequently examined by 7-tesla MRI.Although colloidal iron immersion failed to yield well contrasted images of the colonic epithelium, the HID immersion method clearly delineated the tumor boundary. Contrast ratios differed significantly between cancerous tissue and normal epithelium (P<0.01). Our established ex vivo 7-tesla MRI method combined with HID immersion is a unique approach for delineating the tumor boundary in ESD/EMR specimens.

我们开发了一种使用7特斯拉高分辨率磁共振成像（MRI）结合高铁二胺（HID）浸没的离体成像方法。所有MR图像均使用7特斯拉扫描仪通过稳态（SPGR）梯度回波序列的扰相梯度回忆采集获得。用胶体铁或HID作为杯状细胞粘蛋白的对比剂，对正常大鼠和二甲基肼（DMH）诱发的结肠癌模型大鼠的结直肠标本进行增强，然后用7-T MRI检查，虽然胶体铁浸渍法未能产生结肠上皮的良好对比图像，但HID浸渍法清楚地描绘了肿瘤的边界。癌组织与正常上皮的对比率有显著性差异（P<0.01）。我们建立的离体7特斯拉MRI方法结合HID浸没是一种独特的方法，用于描绘ESD/EMR标本中的肿瘤边界。

项目成果

Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) with hemophagocytosis (HPS): successful treatment using high-dose chemotherapy (BFM-NHL & ALL-90) and autologous peripheral blood stem cell transplantation.

皮下脂膜炎样 T 细胞淋巴瘤 (SPTCL) 伴噬血细胞作用 (HPS)：使用高剂量化疗成功治疗 (BFM-NHL)

• 发表时间: 2013
• 期刊: J Clin Exp Hematop
• 影响因子: 1.5
• 作者: Sakurai E;Satoh T;Akiko YA;Maesawa C;Tsunoda K;Endo M;Akasaka T;Masuda T.
• 通讯作者: Masuda T.