Evaluation of arterial calcification in histology

动脉钙化的组织学评估

• 批准号: 24659340
• 负责人: OSAWA Motoki
• 金额: $ 2.5万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659340/
• 关键词: 循環器; 石灰化; 動脈; ゲノム; 病理学

Arterial calcification is the basic lesion in which aortic dissection and ischemic heart diseases are developed. In the present study, we attempted to establish the histological evaluation procedure of the ectopic calcification. Laboratory staining and immunohistochemistry were performed to sections of paraffin-embedded aorta tissues obtained at autopsy. As the classification, five stages were evident such as diffuse apatite particles, massive calcification accompanied by atheroma, and nucleus or marrow formation. The calcification pattern was not consistent with atherosclerotic changes according to grades of the American Heart Association. In particular, advanced subjects displaying V and VI types exhibited a variety of calcification features. In addition to the common H&E staining, Kossa staining should be suitable at laboratory for evaluation of calcification in the sections. If necessary, immunohistochemistry using anti-MGP and anti-AHSG antibodies should be employed.

动脉钙化是发生主动脉夹层和缺血性心脏病的基本病变。在本研究中，我们试图建立异位钙化的组织学评价程序。实验室染色和免疫组化进行石蜡包埋的主动脉组织切片尸检。分型分为磷灰石颗粒弥漫、大量钙化伴动脉粥样硬化、核或骨髓形成5个阶段。根据美国心脏协会的分级，钙化模式与动脉粥样硬化变化不一致。特别是，显示V型和VI型的晚期受试者表现出各种钙化特征。除了普通的H&E染色外，Kossa染色应适用于实验室评估切片中的钙化。如有必要，应使用抗MGP和抗AHSG抗体进行免疫组化。

项目成果

ゲノムワイドSNPデータに基づく血縁関係推定の試み

尝试根据全基因组 SNP 数据估计亲属关系

• 发表时间: 2013
• 期刊: DNA多型
• 作者: 大澤資樹;稲岡斉彦;田村友紀;坪井秋男;佐藤文子
• 通讯作者: 佐藤文子

HLA typing from sequence data originated by the advanced technology

源自先进技术的序列数据HLA分型

• 发表时间: 2013
• 作者: Osawa M;Suzuki S;Ozaki Y;Ochiai E;Miyashita K;Shiina T.
• 通讯作者: Shiina T.

「死体検案ハンドブック」(改訂3版、2014)

《企业尸检手册》（2014年修订第3版）

• 发表时间: 2014
• 作者: 的場梁次;近藤稔和編
• 通讯作者: 近藤稔和編

Fatal Staphylococcus aureus bacteremia in the Felty syndrome: A maltreatment-suspected case.

费尔蒂综合征中的致命金黄色葡萄球菌菌血症：疑似虐待病例。

• DOI: 10.1016/j.legalmed.2012.03.006
• 发表时间: 2012
• 期刊: Leg Med
• 作者: Satoh F;Seto Y;Hasegawa I;Osawa M
• 通讯作者: Osawa M

Left ventricular non-compaction of myocardium in forensic autopsy

法医尸检左心室心肌致密化不全

• DOI: 10.5897/jcpfm2013.0049
• 发表时间: 2014
• 期刊: J Clin Pathol Forensic Med
• 作者: Satoh F;Nakamura T;Hayashi K;Ochiai E;Osawa M
• 通讯作者: Osawa M