Screening of novel host factors related to HCV lifecycle for developing useful drugs

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A lot of host factors related to infection, replication and release of the hepatitis C virus (HCV) have been reported. However, the whole aspect does not yet become clear. Therefore, using novel gene knockdown methed 'shRNAmir', I tried the exhaustive knockingdown of 35,000 host genes. In this period, I did construction of the required plasmid, establishment of stable cell line, comfirmation of plasmid expression. In addition, I do the exhaustive screening at present. This study allows the identification of the novel host factors of HCV and useful knowledgement for the elucidation of HCV life cycle as well as more effective treatment.