Identification and characterization of hepatic cancer stem cells and cell biological investigation of oncologic process for developing new treatment of HCC

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We investigated to the origin of CSCs and the oncogenic process of HCC. First, we successfully generated oncogene-regulatory expressing transgenic micewhich express the fusion protein of c-Myc and ER. We isolated fetal hepatic progenitor cells from the transgenic fetal mice by the method of cell aggregate which we established previously. And then, we isolated adult hepatocytes from adult transgenic mice. However, it is difficult for us to breed the transgenic mice, so we actually cannot use a needed amount of samples.We are trying to breed and increase the transgenic mice.After that, we will expose hepatocytes at each stage of differentiation to Tamoxifen and acquire cancer cells at several stages.