cartilage regeneration using decellularized tissue, peripheral blood mononuclear cell, and microRNA

本研究的目的是利用脱细胞软骨、外周血单个核细胞和microRNA构建软骨组织。由于不能实现外周血单个核细胞的颗粒培养，因此不可能从外周血单个核细胞诱导软骨形成。用2%SDS脱细胞处理大鼠骨软骨塞12 h，获得脱细胞的含软骨基质的骨软骨塞。但DNA含量并没有被完全去除。接下来，分析软骨细胞片的培养基中的microRNA表达。研究表明，软骨细胞片层分泌的microRNA在软骨形成和骨形成中起着至关重要的作用。

The purpose of this study is to create cartilage tissue using decellularized cartilage, peripheral blood mononuclear cell and microRNA. It was impossible to induce chondrogenesis from peripheral blood mononuclear cell because the pellet culture of peripheral blood mononuclear cell could not be achieved. Osteochondral plug of rat was decellularized by 2%SDS for 12 hours, and decellularized osteochondral plug with cartilage matrix was obtained. But DNA content was not completely removed. Next, microRNA expression in the culture medium of chondrocyte sheet was analyzed. It was revealed that chondrocyte sheet secreted microRNAs which play a crucial role in chondrogenesis and osteogenesis.