Clarification and control of the mechanism of motor and sensory nerve regeneration after transection

1.为探讨条件性损伤对周围神经再生的影响，我们利用背根神经节(DRG)带神经残端的体外组织培养系统，测定了周围神经损伤后感觉神经元轴突的生长情况。结果表明，条件反射效应不仅局限于同侧背根神经节的神经元，而且还影响未受损伤的对侧背根节感觉神经元。此外，我们还研究了条件反射效应是否会影响在体对侧神经的再生。结果表明，损伤后的对侧背根节再生延迟较短，IL-1b和转化生长因子-β1在对侧背根节的表达较高。2.为了阐明感觉神经元和运动神经元在神经再生中的差异，我们观察了坐骨神经切断后GAP-43的表达，证实了…患者前角(AH)细胞中GAP-43的表达增加在背根神经节细胞中，这一点更为明显。此外，我们还对神经再生初期的细节进行了研究。切断并缝合坐骨神经后，在阳性夹部位再次切断坐骨神经，并将近端断端暴露于荧光金(F-G)。F-G阳性标记主要见于DRG细胞，而脊髓AH细胞较少。这些结果提示感觉神经元的再生发生和开始的时间早于运动神经元。3.在硅管中观察远端神经节段体积对神经再生的影响。结果表明，神经再生的程度与远端神经节段的体积无关。我们还检测了给予抗细胞间黏附分子(ICAM-1)和白细胞功能相关抗原(LFA-1)的单抗(MOAB)是否可以促进同种异体小鼠的神经再生。结论：单抗能有效促进同种异体神经移植段的神经再生。4.迄今为止，细胞因子在嵌顿性神经病神经元中的表达尚不清楚。我们观察了DRG细胞在形成和恢复过程中IL-1和TGF-β1的变化以及电生理的变化。IL-1表达达到高峰后，转化生长因子-β1表达达到高峰，此后运动神经传导速度随细胞因子的变化而变化。综上所述，细胞因子在嵌顿性神经病的形成和恢复过程中起着重要的作用。较少

1.To determine the effects of the conditioning lesion on the peripheral nerve regeneration, we measured the axonal outgrowths of sensory neurons in response to peripheral nerve injuries using an in vitro tissue culture system of dorsal root ganglia (DRG) with attached nerve stump. It was concluded that the conditioning effect was not merely restricted to the ipsilateral neurons but also affected undamaged sensory neurons of the contralateral DRG. Furthermore we studied if the conditioning effect affected the nerve regeneration in the contralateral nerves in vivo. In results, the initial delay of regeneration was shorter and the expression of IL-1b and TGF-b1 in the contralateral DRG was higher when the contralateral nerves were previously injured.2.To elucidate the difference in nerve regeneration between sensory neurons and motoneurons, we observed the expression of GAP-43 after transection of the sciatic nerves and confirmed the increased level of GAP-43 in anterior horn (AH) cells f … More ollowed that in DRG cells. In addition, we studied the detail of the initial stage of nerve regeneration. After transected and sutured, the sciatic nerves were retransected at the positive pinch site and exposed the proximal stumps to Fluoro-Gold (F-G). The F-G positive labeling was observed more dominantly in DRG cells than the spinal AH cells. These findings suggested that the regeneration of sensory neurons occurred and started earlier than that of motoneurons.3.The influence of distal nerve segment volume on nerve regeneration in silicon tubes, mass of nerve fragment was examined. The results indicated that the degree of nerve regeneration does not correlate with the volume of distal nerve segment. We also examined whether giving monoclonal antibodies (MoAb) to intercellular adhesion molecule(ICAM-1) and leukocyte function associated antigen (LFA-1) can improve nerve regeneration in allogeneic mice. We conclude that giving MoAb could effectively improve nerve regeneration in grafted allogeneic nerve segments.4.To date, the expression of cytokines in the neurons in the entrapment neuropathy still remains unclear. We observed the changes of IL-1 and TGF-b1 in the DRG cells during both formation and recovery process, together with electrophysiological changes. After expression of IL-1 reached its peak, expression on TGF-b1 peaked and thereafter changes of motor nerve conduction velocity followed the changes of cytokines in the formation as well as the recovery process. In conclusion, cytokines are deeply involved in the formation as well as the recovery process of entrapment neuropathy. Less