Therapeutic strategy for osteoarthritis by evaluation and regulation of microRNA

通过microRNA的评估和调控治疗骨关节炎

• 批准号: 22659273
• 负责人: OCHI Mitsuo
• 金额: $ 1.54万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659273/
• 关键词: 変形性関節症; microRNA; 軟骨; 滑膜; biomarker; biomrker

The purpose of this study is to examine the expression level of miRNA in serum and urine from osteoarthritis(OA) patients and determine its usefulness as biomarker of diagnosis of OA. In addition, secreted miRNA from mesenchymal stromal cells was injected into OA rat, and examine the therapeutic effect. In real time PCR analysis, there is no significant difference of expression of miRNA-146 and miRNA-223 between healthy subjects and OA patients. In animal model, there was no significant therapeutic effect of administration of secreted miRNA from mesenchymal stromal cell in OA rat.

本研究的目的是检测骨关节炎（OA）患者血清和尿液中miRNA的表达水平，并确定其作为OA诊断生物标志物的有用性。另外，将骨髓间充质干细胞分泌的miRNA注射到OA大鼠体内，观察其治疗效果。真实的实时荧光定量PCR检测结果显示，正常人和OA患者的miRNA-146和miRNA-223的表达无显著性差异。在动物模型中，给予从间充质基质细胞分泌的miRNA对OA大鼠没有显著的治疗作用。