An Experimental study on the transplantation of the anterior horn cells of spinal cord to prevent muscular degeneration

脊髓前角细胞移植预防肌肉变性的实验研究

• 批准号: 06454430
• 负责人: OCHI Mitsuo
• 金额: $ 4.86万
• 依托单位: Shimane Medical University (1995)Hiroshima University (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454430/
• 关键词: anterior horn cell; transplantation; muscular degeneration; 筋肉変性予防

Control groupThe right sciatic nerve of inbred Lewis rats was cut at the central region and the distal tip was transected at the muscle entry site.Graft groupAllograft was made of the anterior horn of spinal cord of PVG rats in the TA muscle of the rats treated as described above, following allograft, administration of immunosuppresant (FK506) was made daily.At 4,8,24 weeks, measurement of the wet weight of the TA muscle, twitch tension, and tetanic tension of each group was made, followed by histological examination of the TA muscle. In the graft group, the value of the wet weight, twitch tension, tetanic tension tended to be higher than those in the control group, but the difference was not statistically significant. Furthermore, survival of anterior horn cells of grafted spinal cord grafted in the TA muscle could not also be confirmed histologically. Therefore, in order to examine the condition of the grafted spinal cord cells during the early postoperative period, that is, from 1 day to 1 week postoperatively, histological study was made during the early postoperative period. It was confirmed by this study that necrosis developed in the anterior horn cells grafted in the muscle from the early postoperative period with no survival of these cells. The histological study suggested that allograft of anterior horn cells was not rejected by immuno-response and that the environment surrounding the cells was inadequate for cell survival. AS it was considered that the primary problem point involved was how to achieve survival of the grafted anterior horn cells, study now in progress by grafting fetal spinal cord outstanding nerve regeneration capacity.

对照组：近交系Lewis大鼠右侧坐骨神经中央区切断，远端肌肉切断处切断。移植物组将PVG大鼠脊髓前角移植于同种异体大鼠的TA肌内，移植后每日给予免疫抑制剂(FK506)，于术后4、8、24周测量各组TA肌湿重、抽动张力、强直肌张力，并进行组织学检查。移植物组湿重、抽动张力、强直张力均有高于对照组的趋势，但差异无统计学意义。此外，移植于TA肌的脊髓前角细胞的存活也不能从组织学上得到证实。因此，为了观察移植脊髓细胞在术后早期，即术后1天至1周的情况，在术后早期进行了组织学研究。这项研究证实，从术后早期开始，移植到肌肉中的前角细胞就出现了坏死，这些细胞没有存活。组织学研究表明，同种异体前角细胞移植没有免疫排斥反应，细胞周围的环境不足以使细胞存活。由于目前所涉及的首要问题是如何实现移植的前角细胞的存活，因此通过移植胎儿脊髓具有良好的神经再生能力的研究正在进行中。