An Experimental study on the transplantation of the anterior horn cells of spinal cord to prevent muscular degeneration

对照组自交系Lewis大鼠右侧坐骨神经中央区切开，远端肌入口处横切。移植物组将PVG大鼠脊髓前角移植到上述处理的大鼠TA肌中，移植后每天给予免疫抑制剂FK506。在4、8、24周时测定各组TA肌湿重、抽搐张力、破伤风张力，并对TA肌进行组织学检查。移植物组湿重、抽搐张力、破伤风张力值均有高于对照组的趋势，但差异无统计学意义。此外，移植于TA肌的脊髓前角细胞的存活也不能从组织学上得到证实。因此，为了检查术后早期，即术后1天至1周内移植脊髓细胞的情况，我们在术后早期进行组织学研究。本研究证实，从术后早期开始，移植到肌肉中的前角细胞发生坏死，但这些细胞没有存活。组织学研究表明，同种异体前角细胞移植物没有免疫排斥反应，细胞周围的环境不适合细胞存活。由于认为主要的问题点是如何实现移植的前角细胞的存活，目前正在进行的研究是通过移植具有突出神经再生能力的胎儿脊髓。

Control groupThe right sciatic nerve of inbred Lewis rats was cut at the central region and the distal tip was transected at the muscle entry site.Graft groupAllograft was made of the anterior horn of spinal cord of PVG rats in the TA muscle of the rats treated as described above, following allograft, administration of immunosuppresant (FK506) was made daily.At 4,8,24 weeks, measurement of the wet weight of the TA muscle, twitch tension, and tetanic tension of each group was made, followed by histological examination of the TA muscle. In the graft group, the value of the wet weight, twitch tension, tetanic tension tended to be higher than those in the control group, but the difference was not statistically significant. Furthermore, survival of anterior horn cells of grafted spinal cord grafted in the TA muscle could not also be confirmed histologically. Therefore, in order to examine the condition of the grafted spinal cord cells during the early postoperative period, that is, from 1 day to 1 week postoperatively, histological study was made during the early postoperative period. It was confirmed by this study that necrosis developed in the anterior horn cells grafted in the muscle from the early postoperative period with no survival of these cells. The histological study suggested that allograft of anterior horn cells was not rejected by immuno-response and that the environment surrounding the cells was inadequate for cell survival. AS it was considered that the primary problem point involved was how to achieve survival of the grafted anterior horn cells, study now in progress by grafting fetal spinal cord outstanding nerve regeneration capacity.