Molecular basis of novel estrogen receptor variants expressed in endometriotic tissues

芳香酶基因的上调导致子宫内膜异位症组织中的高雌激素环境。迫切需要澄清的问题是了解雌激素作用的分子基础。到目前为止，高雌激素受体(ER)β和低ERα已被用于解释子宫内膜异位症的病理生理学。我们重新评估了ER在子宫内膜异位症组织中表达的分子基础。检测到4个ERβ变异体的转录本。其中，内质网β5基因最具优势。ERαmRNA在异位内膜细胞中的表达较内膜细胞低7倍。虽然检测到了一个预测野生型66 KDa ERα的单一cDNA序列，但该蛋白的表达是一个55 KDa的分子，这一发现为理解高雌激素在子宫内膜异位症中的病理生理作用提供了一个新的方面。

A maked up-regulation of aromatase gene leads to a hight estrogen environment in endometriotic tissues. The urgent issue to be clarified is to understand the molecular basis of estrogen action. So far, a higher estrogen reseptor (ER) beta and a lower ER alpha endometriotic tissues have been documented to explain the pathophysiology of endometriosis.We re-evaluated the molecular basis of ER expression in endometriotic tissues. Transcripts for 4 ER beta variants were detectable. Among them, the ER beta 5 was the most dominant. ER alpha mRNA the expression in endometriotic cells was 7-fold lower than that in endometrial cells. Although a single cDNA sequence predicting a wild-type 66KDa ER alpha was detectable, the protein expression was found to be a 55KDa molecule.Findings provide a new facet in understanding the pathophysiological role of high estrogen in endometriosis.