Molecular basis of novel estrogen receptor variants expressed in endometriotic tissues

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A maked up-regulation of aromatase gene leads to a hight estrogen environment in endometriotic tissues. The urgent issue to be clarified is to understand the molecular basis of estrogen action. So far, a higher estrogen reseptor (ER) beta and a lower ER alpha endometriotic tissues have been documented to explain the pathophysiology of endometriosis.We re-evaluated the molecular basis of ER expression in endometriotic tissues. Transcripts for 4 ER beta variants were detectable. Among them, the ER beta 5 was the most dominant. ER alpha mRNA the expression in endometriotic cells was 7-fold lower than that in endometrial cells. Although a single cDNA sequence predicting a wild-type 66KDa ER alpha was detectable, the protein expression was found to be a 55KDa molecule.Findings provide a new facet in understanding the pathophysiological role of high estrogen in endometriosis.