Analysis of pathogenisis of metal allergy using humanized murine model

本研究的目的是通过重建小鼠的人体免疫系统来阐明金属过敏的发病机制。我们使用转基因免疫缺陷小鼠作为受体，并尝试将人外周血单个核细胞移植到小鼠体内。我们还减少了小鼠体内的巨噬细胞以增加免疫缺陷。我们观察到人淋巴细胞在小鼠体内的植入非常弱，但不能诱导金属过敏。由于利用人源化小鼠分析金属过敏的发病机制仍然是为下一代医疗服务发展的一个有吸引力的项目，我们将继续实现这一项目。

The aim of this study was to elucidate pathogenesis of metal allergy using humanized mice by reconstituting human immune system in the mice. We used genetically modified immunodeficient mice as recipients and tried to engraft human peripheral blood mononuclear cells in the mice. We also depleted macrophages in the mice to increase immunodeficiency. We observed very weak engraftment of human lymphocytes in the mice but are unable to induce metal allergy. As analysis of pathogenesis of metal allergy using humanized mice is still fascinating project to develop medical service for the next generation, we continue to realize this project.