Activation of human gingival epithelial cells by black-pigmented bacteria
Activation of human gingival epithelial cells by black-pigmented bacteria
批准号:
09671843
负责人:
SUGAWARA Shunji
金额:
$1.92万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998
中文摘要
1. 人牙龈上皮细胞对普雷沃氏菌间质糖蛋白(PGP)和牙龈卟啉单胞菌的菌毛和脂多糖(LPS)部分产生白细胞介素(IL)-8、粒细胞集落刺激因子和粒细胞-巨噬细胞集落刺激因子。相反,在干扰素(IFN)- γ刺激下,细胞产生巨噬细胞集落刺激因子。2 .牙龈芽孢杆菌的PGP、菌毛和LPS组分增强了人牙龈上皮细胞表面细胞间黏附分子-1 (ICAM-1)的表达。通过逆转录酶pcr证实了黑色素细菌(black pigmentation bacteria, BPB)成分对细胞中上述细胞因子和ICAM-1的mRNA诱导作用。人类牙龈上皮细胞不表达主要的LPS受体CD14,这表明细胞被BPB成分激活的途径与肠杆菌科LPS不同。1998年,toll样受体(TLRs)被报道为UPS的信号分子,并且TLRs在高浓度LPS下以不依赖cd14的方式介导LPS信号。tlr通过BPB组分参与人牙龈上皮细胞活化的实验正在进行中。人牙龈上皮细胞具有IL-18,一种ifn - γ诱导因子,在细胞中可能作为前体形式存在。本研究使用的这些刺激剂均不能诱导细胞分泌IL-18。然而,有可能细胞产生的il -18通过某种刺激,如凋亡途径,诱导免疫细胞产生ifn - γ,进而产生ifn - γ参与牙周炎症的发生和治愈。
英文摘要
1. Human gingival epithelial cells produce interleukin (IL)-8, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in response to Prevotella intermedia glycoprotein (PGP), and fimbriae andl ipopolysaccharide (LPS) fraction of Porphyromonas gingivalis. In contrast, the cells produce macrophage-colony stimulating factor stimulated with interferon (IFN)-gamma.2. P.intermedia PGP, fimbriae and LPS fraction of P.gingivalis augmented the expression of intercellular adhesionmolecule-1 (ICAM-1) on the surface of human gingival epithelial cells, flow cytometically.3. mRNA induction of the above cytokines and ICAM-1 in the cells by the components of black-pigmented bacteria (BPB)was confirmed by reverse-transcriptase PCR.4. Human gingival epithelial cells do not express CD14, a major LPS receptor, which indicates that the cells are activated by the components of BPB with a distinct pathway from LPS of Enterobacteriaceae. In 1998, Toll-like receptors (TLRs)were reported to be signaling molecules of UPS, and also TLRs mediated LPS signal in a CD14-independent manner at a high concentration of LPS.The experiment that TLRs participate in the activation of human gingival epithelial cells by components of BPB is under way.5. Human gingivalepithelial cells possess IL-18, a IFN-gamma inducing factor, in the cells as a possibly precursor form. Thes timulants used in this study could not induce the secretion of IL-18 by the cells. However, it is possible that IL-18produced by the cells with some stimulation, such as apoptotic pathway may induce IFN-gamma by immune cells, and that in turn, IFN-gamma produced may participate in the onset and cure of inflammation in periodontium.
期刊论文(0)
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科研奖励(0)
会议论文
Sugawara, S.: "Heterogeneous expression and release of CD14 by human gingival fibroblasts : Characterization CD14-mediated interleukin-8 secretion in response to lipopolysaccharide." Infection and Immunity. 66-7. 3043-3049 (1998)
Sukawara, S.:“人类牙龈成纤维细胞 CD14 的异质表达和释放:表征 CD14 介导的白细胞介素 8 分泌对脂多糖的反应。”
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期刊:
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作者:
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通讯作者:
Basic research for development of effective inducing strategy of sublingual immune tolerance
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