Dvelopment of novel adjuvant which constructed by DNA origami technology

近年来，病原微生物获得了耐药性，并在世界范围内蔓延。抗生素在世纪被认为是治疗感染性疾病的“灵丹妙药”，然而抗生素的大量使用导致了耐药微生物的出现。因此，在发展中国家，用基本抗生素治疗大多数婴儿腹泻变得困难。目前，在基础研究的基础上，开发和完善肠源性和全身性免疫激活剂具有重要意义。在这个提议中，我们专注于DNA序列中的CpG基序。CpG基序在病原微生物的基因组中以高频率观察到。它作为一种免疫激活剂，促进抗原特异性免疫反应的产生。本研究以M13 DNA为载体，构建了将GpG基序固定在体外的“DNA折纸”免疫激活剂，旨在获得免疫增强效果。

Recently, pathogenic microorganisms acquired drug-resistance, and they are spreading throughout the world. Antibiotics were presented as "magic bullets" against infectious diseases in the 20th century, however, the heavy usage of antibiotics has led to the appearance of drug-resistant microorganisms. Thus, it has become difficult to treat most infantile diarrhea with basic antibiotics in the developing countries. Now, it is important to develop and improve the enteric and general immune activators on the basis of fundamental investigations. In this proposal, we focus on a CpG motif among the DNA sequence. The CpG motif is observed at high frequency in the genomes of pathogenic microorganisms. It works as an immune activator to boost production of antigen-specific immune responses. In this study, we constructed "DNA origami" immune activators being fixed the GpG motifs to the outside using M13 DNA, aiming to obtain the immunopotentiating effect.