Multi-dimensional analyses of a novel immune system NETs-inducer using nano live imaging technology

利用纳米实时成像技术对新型免疫系统NETs诱导剂进行多维分析

• 批准号: 23390419
• 负责人: TERAO Yutaka
• 金额: $ 12.15万
• 依托单位: Niigata University (2012-2013)Osaka University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390419/
• 关键词: レンサ球菌; NETs; 免疫回避; 菌体表層タンパク

Recently, it was reported that neutrophils equips a novel immune system against pathogenic microorganisms. And then, it was named neutrophils extracellular traps, NETs. NETs work to trap the pathogenic bacteria and to present toward macrophages and other native neutrophils. However, they were still unknown what molecules derived from pathogens induces NETs, and what receptor on neutrophil recognize NETs-inducing molecule. Thus, we investigated to determine the molecules which induce NETs formation, and then how neutrophils recognize the NETs-inducing factor in this study. In addition, we examine the NETs-expressing neutrophils could eliminate major pathogenic bacteria, including Streptococcus pneumoniae, Streptococcus pyogenes, and Staphilococcus aures in vitro assys.

最近，有报道称中性粒细胞装备了一种新的免疫系统来对抗病原微生物。然后，它被命名为中性粒细胞细胞外陷阱，NETs。net的作用是捕获致病菌，并向巨噬细胞和其他天然中性粒细胞呈现。然而，目前尚不清楚哪些来自病原体的分子诱导NETs，以及中性粒细胞上哪些受体识别NETs诱导分子。因此，我们在本研究中研究了诱导NETs形成的分子，以及中性粒细胞如何识别NETs诱导因子。此外，我们还在体外检测了表达nets的中性粒细胞能够清除主要致病菌，包括肺炎链球菌、化脓性链球菌和金黄色葡萄球菌。

项目成果

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