Multi-dimensional analyses of a novel immune system NETs-inducer using nano live imaging technology

最近，据报道，中性粒细胞装备了一种新的免疫系统，以对抗病原微生物。然后，它被命名为中性粒细胞胞外陷阱，NETs。NET的工作是捕获致病细菌，并向巨噬细胞和其他天然中性粒细胞呈递。然而，目前尚不清楚病原体来源的哪些分子诱导NETs，以及中性粒细胞上的哪些受体识别NETs诱导分子。因此，我们研究确定诱导NETs形成的分子，然后在这项研究中中性粒细胞如何识别NETs诱导因子。此外，我们在体外试验中检测了表达NETs的中性粒细胞可以清除主要的致病菌，包括肺炎链球菌、化脓性链球菌和金黄色葡萄球菌。

Recently, it was reported that neutrophils equips a novel immune system against pathogenic microorganisms. And then, it was named neutrophils extracellular traps, NETs. NETs work to trap the pathogenic bacteria and to present toward macrophages and other native neutrophils. However, they were still unknown what molecules derived from pathogens induces NETs, and what receptor on neutrophil recognize NETs-inducing molecule. Thus, we investigated to determine the molecules which induce NETs formation, and then how neutrophils recognize the NETs-inducing factor in this study. In addition, we examine the NETs-expressing neutrophils could eliminate major pathogenic bacteria, including Streptococcus pneumoniae, Streptococcus pyogenes, and Staphilococcus aures in vitro assys.