G-Protein-coupled receptors in endothelial cells: from single-cell expression to new functions (A12*)

内皮细胞中的 G 蛋白偶联受体：从单细胞表达到新功能 (A12*)

• 批准号: 397830024
• 金额: --
• 依托单位: Abteilung II: Pharmakologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/397830024?language=en
• 关键词: Protein; coupled; receptors; endothelial; cells

G-protein-coupled receptors (GPCRs) mediate the intracellular effects of a multitude of systemic and local mediators and thereby importantly modulate endothelial function. To define new roles for GPCRs in endothelial cells (EC), we performed a single-cell GPCR expression analysis in freshly isolated murine EC from different vascular beds and disease states. We identified a number of GPCRs selectively expressed in inflamed EC from brain, aorta and lung. Focusing on three orphan GPCRs that have not yet been implicated in endothelial biology, we here will analyze these orphan receptors in vitro and in tamoxifen-inducible, EC-specific knockout mice in vivo.

G蛋白偶联受体（GPCR）介导大量全身和局部介质的细胞内效应，从而重要地调节内皮功能。为了确定GPCR在内皮细胞（EC）中的新作用，我们在来自不同血管床和疾病状态的新鲜分离的小鼠EC中进行了单细胞GPCR表达分析。我们鉴定了许多GPCR选择性地在来自脑、主动脉和肺的发炎EC中表达。集中在三个孤儿GPCR尚未牵连在内皮生物学，我们在这里将分析这些孤儿受体在体外和他莫昔芬诱导，EC特异性基因敲除小鼠体内。