G-Protein-coupled receptors in endothelial cells: from single-cell expression to new functions (A12*)

g蛋白偶联受体（gpcr）介导多种全身和局部介质的细胞内效应，从而重要地调节内皮功能。为了确定GPCR在内皮细胞（EC）中的新作用，我们对来自不同血管床和疾病状态的新鲜分离的小鼠EC进行了单细胞GPCR表达分析。我们在脑、主动脉和肺的炎症EC中发现了一些选择性表达的gpcr。以尚未涉及内皮生物学的三种孤儿gpcr为重点，我们将在体外和他莫昔芬诱导的ec特异性敲除小鼠体内分析这些孤儿受体。

G-protein-coupled receptors (GPCRs) mediate the intracellular effects of a multitude of systemic and local mediators and thereby importantly modulate endothelial function. To define new roles for GPCRs in endothelial cells (EC), we performed a single-cell GPCR expression analysis in freshly isolated murine EC from different vascular beds and disease states. We identified a number of GPCRs selectively expressed in inflamed EC from brain, aorta and lung. Focusing on three orphan GPCRs that have not yet been implicated in endothelial biology, we here will analyze these orphan receptors in vitro and in tamoxifen-inducible, EC-specific knockout mice in vivo.