Development of a new pretreatment method for plasma endotoxin assay

1.我们设计了一种新的前处理方法(新的主成分分析方法)，可以进行总内毒素的测定。用内毒素专门法测定人血浆中的含量。该方法可用于革兰氏阴性细菌败血症的早期快速诊断。利用这种方法，结合内毒素特异的凝胶试验，研究了内毒素与脓毒症、感染性休克、胃肠道穿孔、烧伤、弥散性血管内凝血(DIC)等症状的关系。和多器官衰竭。并对其进行了澄清。发现内毒素和多种细胞因子是上述症状发生的触发因素。很多研究人员，临床医生。日本和国外的临床检查员都要求我们使用这种方法，所以我们正在使这种方法可行。该方法是诊断深部真菌病的较好方法。因此，我们相信这种方法将有助于拯救人类的生命。

1. A new pretreatment method(the new PCA method), which we devised, permitted a measurement of the total endotoxin. content in human plasma by an endotoxin-spec'tic assay. This method was found to be useful for the early and rapid diagnosis of gram-negative bacterial sepsis.2. Using this method, combined with an endotoxin-specific limulus test, relation of endotoxin with symptoms such as sepsis, septic shock, gastrointestinal tract perforation, burns, DIC(disseminated intravascular coagulation). and multiple organ failure. was elucidated.3. It was found that endotoxin and many cytokines were concerned as triggers for the initiation of these symptoms mentioned above.4. Many researchers, clinicians. and clinical inspectors of Japan and, foreign countries are asking us to use this method, so we are making this method available.5. This method was applied as the superior method for diagnosis of deep-seated mycosis.6. Consequently, we believe this method will contribute to saving human lives.