Development of a new pretreatment method for plasma endotoxin assay

血浆内毒素检测前处理新方法的研制

• 批准号: 01870116
• 负责人: ENDO Shigeatsu
• 金额: $ 4.8万
• 依托单位: Critical Care and Emergency Center, School of Medicine, Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01870116/
• 关键词: Endotoxemia; Glucan; Plasma pretreatment; Limulus test; Sepsis; Rapid diagnosis; グラト陰性菌敗血症; 深在性真菌症; 新過塩素酸処理法; 発色合成基質法

1. A new pretreatment method(the new PCA method), which we devised, permitted a measurement of the total endotoxin. content in human plasma by an endotoxin-spec'tic assay. This method was found to be useful for the early and rapid diagnosis of gram-negative bacterial sepsis.2. Using this method, combined with an endotoxin-specific limulus test, relation of endotoxin with symptoms such as sepsis, septic shock, gastrointestinal tract perforation, burns, DIC(disseminated intravascular coagulation). and multiple organ failure. was elucidated.3. It was found that endotoxin and many cytokines were concerned as triggers for the initiation of these symptoms mentioned above.4. Many researchers, clinicians. and clinical inspectors of Japan and, foreign countries are asking us to use this method, so we are making this method available.5. This method was applied as the superior method for diagnosis of deep-seated mycosis.6. Consequently, we believe this method will contribute to saving human lives.

1.我们设计了一种新的预处理方法（新的PCA方法），允许测量总内毒素。通过内毒素特异性分析测定人血浆中的含量。该方法可用于革兰阴性菌败血症的早期快速诊断.用此方法结合内毒素特异性鲎试验，可检测内毒素与脓毒症、感染性休克、胃肠道穿孔、烧伤、DIC（弥散性血管内凝血）等症状的关系。和多器官衰竭阐明了3.发现内毒素和多种细胞因子与上述症状的发生有关.许多研究人员，临床医生。日本和外国的临床检查人员要求我们使用这种方法，所以我们正在使用这种方法。该方法是诊断深部真菌病的上级方法.因此，我们相信这种方法将有助于拯救人类生命。

项目成果

Shigeatsu Endo: "Small dese intramuscular injection and oral administration of pliymyxin B for endotoxemia." Archives of Emergency Medicine.

Shigeatsu Endo：“小剂量肌肉注射和口服百粘菌素 B 治疗内毒素血症。”

Shigeatsu Endo: "Evaluation of IPM/CS in Gramーnegative bacillces infections:use of endotoxinーspecific assay" Clin.Therapent.12. 352-356 (1990)

Shigeatsu Endo：“革兰氏阴性杆菌感染中 IPM/CS 的评估：内毒素特异性测定的使用”Clin.Therapent.12 (1990)。

遠藤重厚: "真菌による敗血症に対するMiconazoleの7著効例ーToxicolorによる迅速診断法を用いて" 基礎と臨床. 23. 153-157 (1989)

Shigetoshi Endo：“咪康唑治疗真菌引起的脓毒症的七例有效病例 - 使用 Toxicolor 的快速诊断方法”基础与临床实践。 23. 153-157 (1989)。

K. Inada, et al.: "Establishment of a New Perchloric Acid Treatment Method to Allow Detection of the Total Endotoxin Contentin Human Plasma by the Limulus Test and Clinical Application." Microbiol. Immuno 1.35. 303-314 (1991)

K. Inada 等人：“建立一种新的高氯酸处理方法，通过鲎试验检测人血浆中的总内毒素含量并进行临床应用。”

S. Endo, et al: "Endotoxin (LPS) and host responses (2)" Med. Postgrad.30. 27-34 (1992)

S. Endo 等人：“内毒素 (LPS) 和宿主反应 (2)”Med。