Dualer, gepulster optisch-parametrischer Oszillatorlaser

申请资金用于使用泵浦探针激励用于光声成像和光谱学的定制双光学参量振荡器（OPO）激光系统。与传统的OPO激光器相比，该系统将在可见光和近红外波长区域提供独立控制的、波长可调的激发，脉冲之间的可变时间延迟（从1 ns到1000 ns），快速（脉冲对脉冲）波长调谐，高脉冲能量（> 70 mJ）和高重复率（200 Hz）。这不仅将使新型造影剂和基因表达报告蛋白的开发、综合评估和进一步完善成为可能，还将为该技术在临床前和长期临床研究中的体内应用提供基础。双OPO激光器将克服目前可用的OPO激光系统所施加的限制。

Funding is requested for a custom-made dual optical parametric oscillator (OPO) laser system for photoacoustic (PA) imaging and spectroscopy using pump-probe excitation. In contrast to conventional OPO lasers, this system will provide independently controlled, wavelength-tuneable excitation across the visible and near-infrared wavelength region, variable time delays (from 1 ns to 1000 ns) between the pulses, fast (pulse-to-pulse) wavelength tuning, high pulse energies (> 70 mJ) and high repetition rates (200 Hz). This will not only enable the development, comprehensive evaluation and further refinement of novel contrast agents and genetically expressed reporter proteins for PA imaging in vitro but will also provide the basis for the translation of this technology to in vivo applications in preclinical and, in the longer term, clinical studies. A dual-OPO laser will overcome the limitations imposed by the OPO laser systems currently available to our group.