Direct conversion of plant biomass to isoprene by microorganisms expressing cell-wall degrading enzymes from parasitic plants

点击翻译按钮获取中文摘要

Enzymes which can degrade cell-wall polysaccharides of plants are useful for effective conversion of plant biomass. In this project, we focused on a parasitic plant, Orobanche minor, which can degrade the cell-wall of roots of their host plants. From RNAseq data, a beta-glucosidase homologous gene was obtained. The enzyme expressed in E. coli showed beta-mannosidase activity when cell-wall polysaccharides from O. minor seeds were used as a substrate. This is the first report that indicates beta-mannosidase from plants showed activity toward plant cell-wall components.