Analysis of Interactions in High Molecular Weight Protein Complexes by Using Segment Label and Cutting-edge NMR Technologies

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To develop new methodologies for structural analysis of biologically important high-molecular-weight protein complexes, applications of the segment label and new NMR techniques such as the transfer cross-saturation (TCS) and residual dipole coupling (RDC) were examined. By using TCS for the final electron transfer complex in the respiratory chain, the complex between cytochrome c and cytochrome c oxidase, we successfully determined the amino acid residues of cytochrome c directly interacting with cytochrome c oxidase.