Structural Characterization of Heme-mediated Signaling Mechanism in Protein Regulation System

蛋白质调节系统中血红素介导的信号机制的结构表征

• 批准号: 21370040
• 负责人: ISHIMORI Koichiro
• 金额: $ 12.15万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370040/
• 关键词: ヘム依存性制御蛋白質; 酸化修飾; 活性酸素; ヘム生合成; 鉄代謝

In this research project, we characterized the heme binding in Iron Response Regulator (Irr) and Iron Regulatory Protein (IRP) and examined their functional significance. We spectroscopically identified the heme binding sites of Irr and, based on the structural information, a molecular mechanism of heme-induced oxidative modification in Irr was proposed. The heme binding sites of IRP were also determined and a new mechanism for the translational regulation by binding of heme was suggested in IRP. These new findings in this research project shed new light on the functional significance of heme in vivo.

在本研究项目中，我们表征了铁反应调节因子（Irr）和铁调节蛋白（IRP）中的血红素结合，并研究了它们的功能意义。我们光谱鉴定的血红素结合位点的Irr，并根据结构信息，血红素诱导的氧化修饰的分子机制，Irr提出。还确定了IRP的血红素结合位点，并提出了IRP中通过血红素结合进行翻译调控的新机制。本研究项目中的这些新发现为血红素在体内的功能意义提供了新的线索。

项目成果

Molecular oxygen regulates the enzymatic activity of a heme-containing diguanylate cyclase (HemDGC) for the synthesis of cyclic di-GMP.

• DOI: 10.1016/j.bbapap.2009.09.028
• 发表时间: 2010
• 期刊: Biochimica et biophysica acta
• 作者: H. Sawai;S. Yoshioka;T. Uchida;M. Hyodo;Y. Hayakawa;K. Ishimori;S. Aono

Unusual Heme Binding in the Bacterial Iron Response Regulator Protein (Irr) : SpectralCharacterization of Heme Binding to HemeRegulatory Motif.

细菌铁反应调节蛋白 (Irr) 中异常的血红素结合：血红素与血红素调节基序结合的光谱表征。

• 发表时间: 2011
• 期刊: Biochemistry 50
• 作者: Ishikawa;H.;Nakagaki;M.;Bamba;A.;Uchida T.;Hori;H.;O'Brian;M.R.;Iwai;K.;Ishimori;K.
• 通讯作者: K.

A Heme Degradation Enzyme, HutZ, fromVibrio cholera

来自霍乱弧菌的血红素降解酶 HutZ

• 发表时间: 2012
• 期刊: Chem. Comm.
• 作者: Uchida;T.;Sekine;Y.;Matsui;T.;Ikeda-Saito;M.;Ishimori;K.
• 通讯作者: K.

Oxygen Activation and Oxidative Modification in Heme-regulated Proteins

血红素调节蛋白中的氧活化和氧化修饰

• 发表时间: 2011
• 作者: Motomura;H.;M.-A. Selosse;F. Martos;A. Kagawa & T. Yukawa;石森浩一郎
• 通讯作者: 石森浩一郎

鉄代謝制御タンパク質IRP2の機能発現における酸化修飾機構

铁代谢调节蛋白IRP2功能表达的氧化修饰机制

• 发表时间: 2011
• 作者: Bhandari;S.;Paudayal;K.N.;Momohara;A.;宮地祐樹
• 通讯作者: 宮地祐樹