Molecular analysis of sex difference in the hippocampus, a center for learning and memory

海马体（学习和记忆中心）性别差异的分子分析

• 批准号: 23657098
• 负责人: KAWATO Suguru
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657098/
• 关键词: 海馬の性差; 神経ステロイド; 脳ステロイドの性差; 記憶の性差; シナプスの性差; 性差; スパインの性差; 海馬; 雌雄差; 記憶

The goal of the current study is elucidation of sex difference in the hippocampus, a center for learning and memory. (1) Concentration of sex hormones in the hippocampus exhibited clear sex difference. Hippocampal concentration of estradiol (E2), the most potent estrogen, was higher in male than that in female. Hippocampal E2 fluctuated across the estrous cycle. The concentration of hippocampal testosterone (T) was higher in male than female, which did not fluctuate. (2) Surprisingly, the expression level of the steroidogenic enzymes and receptors in the hippocampus exhibited neither sex difference nor fluctuation across the estrous cycle.(3) The density of dendritic spines in female hippocampus fluctuated across the estrous cycle. The total spine density showed the maximum at Pro and D1, and the minimum at Est and D2 whereas the total spine density in male was as high as that in female at Pro or D1. The fluctuating pattern of spine density corresponded to that of hippocampal concentration of sex steroids. Taken together, there is clear sex difference in the hippocampus-synthesized sex hormones which play a crucial role in regulating the density of dendritic spines

目前研究的目标是阐明海马体的性别差异，海马体是学习和记忆的中心。(1)海马性激素浓度存在明显的性别差异。雌二醇（E2）是最有效的雌激素，雄性海马浓度高于雌性。海马体E2在发情周期中波动。海马睾酮(T)浓度男性高于女性，且无波动。(2)令人惊讶的是，海马中甾体生成酶和受体的表达水平没有性别差异，也没有在发情周期中波动。(3)雌性海马树突棘密度在整个发情周期中存在波动。总脊柱密度在Pro和D1处最大，Est和D2处最小，而男性的总脊柱密度在Pro和D1处与女性相同。脊柱密度的波动模式与海马性类固醇浓度的波动模式相对应。综上所述，海马体合成的性激素在调节树突棘的密度方面起着至关重要的作用，在这方面存在明显的性别差异

项目成果

De novo synthesized estradiol protects against methylmercury-induced neurotoxicity in cultured rat hippocampal slices.

• DOI: 10.1371/journal.pone.0055559
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Yamazaki T;Yamamoto M;Ishihara Y;Komatsu S;Munetsuna E;Onizaki M;Ishida A;Kawato S;Mukuda T
• 通讯作者: Mukuda T

Mathematical and automated analysis of dendritic spines from confocal laser microscopy images

从共焦激光显微镜图像中对树突棘进行数学和自动分析

• 发表时间: 2012
• 作者: H. MUKAI,;S. KAWATO
• 通讯作者: S. KAWATO

記憶・学習中枢海馬に性差はあるか?その3

海马体（记忆和学习中心）是否存在性别差异？

• 发表时间: 2011
• 作者: Xu;Q.;et al;K. Kato;北條泰嗣,肥後心平,上林正修,向井秀夫,木本哲也,川戸佳
• 通讯作者: 北條泰嗣,肥後心平,上林正修,向井秀夫,木本哲也,川戸佳

Estradiol Rapidly Rescues Synaptic Transmission from Corticosterone-induced Suppression via Synaptic/Extranuclear Steroid Receptors in the Hippocampus

• DOI: 10.1093/cercor/bhr164
• 发表时间: 2012-04-01
• 期刊: CEREBRAL CORTEX
• 影响因子: 3.7
• 作者: Ooishi, Yuuki;Mukai, Hideo;Kawato, Suguru
• 通讯作者: Kawato, Suguru

Endogenous synthesis of corticosteroids in the hippocampus.

• DOI: 10.1371/journal.pone.0021631
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Higo S;Hojo Y;Ishii H;Komatsuzaki Y;Ooishi Y;Murakami G;Mukai H;Yamazaki T;Nakahara D;Barron A;Kimoto T;Kawato S
• 通讯作者: Kawato S