An approach toward establishing Agrobacterium-mediated transformation system of mitochondria

为了建立植物线粒体转化体系，本研究利用农杆菌介导法将T-DNA导入植物线粒体。为了将T-DNA导入线粒体，我们去除了VirD 2中通常起到将T-DNA导入细胞核作用的核定位信号序列，并添加了线粒体靶向信号序列。最后，我们产生了携带修饰的VirD 2与线粒体靶向信号序列的农杆菌。

To establish transformation system of plant mitochondria, we aimed to produce genetically modified Agrobacterium so that it delivered T-DNA into mitochondria. To deliver T-DNA into mitochondria, we removed the nuclear localization signal sequence from VirD2, which usually played a role to deliver T-DNA into nucleus, and added a mitochondrial targeting signal sequence. Finally we produced Agrobacterium carrying modified VirD2 with mitochondrial targeting signal sequence.