Analysis of molecular basis of pollen wall formation and communication signals of pollen

利用外壁或花粉被层形成缺陷的拟南芥突变体，以及表现自交不亲和性和种间不亲和性的油菜物种，我们计划鉴定与外壁和花粉被层形成有关的基因，以及与花粉脱水、自交不亲和性和种间不亲和性等柱头相互作用的信号。我们的研究表明，作为外壁的支架的Primexine和作为外壁的一列的bacula的形成是受孢子生理调节的。我们已经发表了几篇原创论文和评论，其中一篇特邀评论发表在《国际植物发育生物学杂志》上。我们报道了拟南芥花粉外壁模式的形成依赖于三个主要的发育过程，即孢粉合成、初生外植体形成和愈伤组织形成。至于花粉和柱头之间的相互作用，我们证明了区域III和IV对于SP11的识别专一性是重要的，SP11是决定自交不亲和的花粉决定因素。我们还发现种间不亲和性是一个数量性状，因此开发了用于QTL分析的原始DNA标记。我们已经准备好绘制这一特征的图谱。

Using Arabidopsis mutants defective in exine or pollen coat formation as well as using Brassica species showing self-incompatibility and interspecific incompatibility, we are planning to identify genes involved in exine and pollen coat formation and signals for pollen dehydration, interaction between pollen and stigmas such as self-incompatibility and interspecific incompatibility.Our study have demonstrated that formation of primexine, that is a scaffold of exine, and bacula, that is a column of exine, is sporophytically regulated. We have published several original papers and reviews, one of which an invited review in International Journal of Plant Developmental Biology. We report that pollen exine pattern formation is dependent on three major developmental processes, sporopollenin synthesis, primexine formation and callose formation, in Arabidopsis thaliana. We also reports application aspects for plant Breeding in a Japanese journal, Breeding Research.As for the interaction between pollen and stigma, we demonstrated that region III and IV is important for recognition specificity of SP11, which is a pollen determinant of self-incompatibility. We also found that interspecific incompatibility is a quantitative trait and, therefore, developed original DNA markers for QTL analysis. We are ready for mapping of this trait.