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Analysis of molecular basis of pollen wall formation and communication signals of pollen

花粉壁形成的分子基础及花粉通讯信号分析

基本信息

批准号：
16380002
负责人：
TORIYAMA Kinya
金额：
$ 9.73万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380002/
关键词：
pollen signal exine pollen coat self-incompatibility Arabidopsis male sterility

项目摘要

Using Arabidopsis mutants defective in exine or pollen coat formation as well as using Brassica species showing self-incompatibility and interspecific incompatibility, we are planning to identify genes involved in exine and pollen coat formation and signals for pollen dehydration, interaction between pollen and stigmas such as self-incompatibility and interspecific incompatibility.Our study have demonstrated that formation of primexine, that is a scaffold of exine, and bacula, that is a column of exine, is sporophytically regulated. We have published several original papers and reviews, one of which an invited review in International Journal of Plant Developmental Biology. We report that pollen exine pattern formation is dependent on three major developmental processes, sporopollenin synthesis, primexine formation and callose formation, in Arabidopsis thaliana. We also reports application aspects for plant Breeding in a Japanese journal, Breeding Research.As for the interaction between pollen and stigma, we demonstrated that region III and IV is important for recognition specificity of SP11, which is a pollen determinant of self-incompatibility. We also found that interspecific incompatibility is a quantitative trait and, therefore, developed original DNA markers for QTL analysis. We are ready for mapping of this trait.

利用拟南芥外壁和花粉外壳形成缺陷的突变体以及表现自交不亲和和种间不亲和的芸苔属植物，我们计划鉴定与外壁和花粉外壳形成有关的基因以及花粉脱水、花粉与柱头相互作用（如自交不亲和和种间不亲和）的信号。我们的研究表明，原胺的形成，即外壁支架，和杆状体，即外壁柱，是由孢子生理调节的。我们发表了多篇原创论文和综述，其中一篇被《International Journal of Plant Developmental Biology》特邀发表。我们报道了拟南芥花粉外叶模式的形成依赖于三个主要的发育过程：孢粉素合成、原质形成和胼胝质形成。我们还在日本期刊《育种研究》上报道了植物育种的应用方面。在花粉与柱头的相互作用方面，我们证明了III区和IV区对SP11的识别特异性很重要，SP11是花粉自交不亲和的决定因素。我们还发现种间不亲和性是一种数量性状，因此开发了用于QTL分析的原始DNA标记。我们已经准备好对这个特性进行映射了。