Analysis of proteins specifically produced in the tissues during aerenchyma formation in maize

通气组织使芽和根之间的内部通气，因此它的形成对植物适应过量水分环境很重要。我研究了用激光显微解剖（LM）从玉米根部分离的皮层进行综合蛋白质组分析的最佳条件。我确定了组织固定的最佳固定物。此外，我用lm分离的皮质蛋白进行western blotting检测乙醇脱氢酶蛋白。然而，我未能获得足够的蛋白质，以进行全面的蛋白质组分析。

Aerenchyma enables internal aeration between shoots and roots, and its formation is therefore important for the adaptation of plants to excess water environments. I examined optimal conditions for comprehensive proteome analysis using the cortex isolated by laser microdissection (LM) from maize roots. I determined an optimal fixative for fixation of tissues. Moreover, I detected Alcohol dehydrogenase protein by western blotting using LM-isolated cortex proteins. However, I failed to obtain enough amounts of proteins that are required for comprehensive proteome analysis.