Analysis of DNA methylation in the reproductive cells using a tiling array

使用平铺阵列分析生殖细胞中的 DNA 甲基化

• 批准号: 19380004
• 负责人: NAKAZONO Mikio
• 金额: $ 13.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380004/
• 关键词: レーザーマイクロダイセクション; DNAメチレーション; イネ; 生殖系列; DNAメチル化; タイリングアレイ; LMD

In this research, we tried to investigate DNA methylation pattern in laser microdissection (LMD)-isolated embryo and endosperm by a tiling array. We examined optimal conditions for bisulfite sequencing using total DNA extracted from some tissues in rice. We succeeded in the bisulfite sequencing when we used enough amounts of DNA, but failed in the sequencing using small amounts of DNA extracted from the LMD isolated tissues. As a result, we did not succeed in solution of this problem, and thus were not able to carry out the tiling array. We are continuing to examine the optimal conditions for the bisulfite sequencing using small amounts of DNA.

本研究利用激光显微切割技术，采用平铺阵列技术研究了离体胚和胚乳的DNA甲基化模式。我们研究了从水稻某些组织中提取的总DNA进行亚硫酸氢盐测序的最佳条件。当我们使用足够量的DNA时，我们成功地进行了亚硫酸氢盐测序，但使用从LMD分离的组织中提取的少量DNA测序失败。结果，我们没有成功地解决这个问题，因此无法进行平铺阵列。我们正在继续研究使用少量DNA进行亚硫酸氢盐测序的最佳条件。

项目成果

Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate

• DOI: 10.1007/s00299-008-0583-0
• 发表时间: 2008-10-01
• 期刊: PLANT CELL REPORTS
• 影响因子: 6.2
• 作者: Fujimoto, Masaru;Arimura, Shin-ichi;Tsutsumi, Nobuhiro
• 通讯作者: Tsutsumi, Nobuhiro

イネ reduced adh activity (rad) 変異体の子葉鞘における細胞分裂・伸長の抑制

抑制水稻胚芽鞘细胞分裂和伸长，降低 ADH 活性 (rad) 突变体

• 发表时间: 2009
• 作者: 高木俊;土岐和多瑠;吉岡明良;高橋宏和
• 通讯作者: 高橋宏和

A method for obtaining high integrity RNA from developing aleurone cells and starchy endosperm in rice (Oryza saliva L.) by laser microdissection.

一种通过激光显微切割从水稻 (Oryza saliva L.) 发育中的糊粉细胞和淀粉胚乳中获取高完整性 RNA 的方法。

• 发表时间: 2007
• 期刊: Plant Sci. 173
• 作者: Ishimaru;T.;M.Nakazono;T.Masumura;M.Abiko;Y.San-oh;N.K.Nishizawa;M.Kondo
• 通讯作者: M.Kondo

Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death.

• DOI: 10.1266/ggs.82.361
• 发表时间: 2007-08
• 期刊: Genes & genetic systems
• 影响因子: 1.1
• 作者: Haifang Yan;H. Saika;M. Maekawa;I. Takamure;N. Tsutsumi;J. Kyozuka;M. Nakazono

Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission

• DOI: 10.1111/j.1365-313x.2009.03786.x
• 发表时间: 2009-05-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Fujimoto, Masaru;Arimura, Shin-ichi;Tsutsumi, Nobuhiro
• 通讯作者: Tsutsumi, Nobuhiro