Analysis of DNA methylation in the reproductive cells using a tiling array

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In this research, we tried to investigate DNA methylation pattern in laser microdissection (LMD)-isolated embryo and endosperm by a tiling array. We examined optimal conditions for bisulfite sequencing using total DNA extracted from some tissues in rice. We succeeded in the bisulfite sequencing when we used enough amounts of DNA, but failed in the sequencing using small amounts of DNA extracted from the LMD isolated tissues. As a result, we did not succeed in solution of this problem, and thus were not able to carry out the tiling array. We are continuing to examine the optimal conditions for the bisulfite sequencing using small amounts of DNA.