Analysis of transporters and transcription factors controlling suberization in Casparian strip in rice

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In rice, the hypodermis in the outer part of roots forms a suberized cell wall (Casparian strip and/or suberin lamellae), which inhibits the flow of water and ions. We examined if an ABC transporter RCN1 is involved in the Casparian strip and suberin lamellae formations and tried to understand the mechanism of suberization in Casparian strip and suberin lamellae by identifying transcription factors, which control expressions of suberin biosynthetic genes and suberin transporter genes. Under stagnant deoxygenated conditions, well-suberized hypodermis developed in the wild type but not in the rcn1 mutant. An apoplastic tracer, periodic acid, was blocked at the hypodermis in the wild type but not in rcn1, indicating that the apoplastic barrier in the mutant was impaired. Moreover, we identified some transcription factors, which might control the formations of Casparian strip and suberin lamellae.