Identification of genes conferring tolerance to submergence or waterlogging stress and generation of submergence-tolerant crops

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Rapid environmental changes subject plants to various abiotic stresses that adversely affect their growth and development. In Japan, crops (e.g., soybean, barley and wheat) are often deprived of oxygen by floods and heavy rainfall in rainy season. Because plants are sessile, their ability to respond quickly to the waterlogged or submergence stress is crucial for adaptation arid survival. To identify genes that can confer waterlogging or submergence tolerance to crops, in this project, we screened the mutants that show more tolerance to submergence using the activation-tagged mutant library and the EMS-mutagenized mutant library of Arabidopsis thaliana. Finally, we selected two mutant lines from 310,000 activation tagged lines and determined the nucleotide sequences at the border of T-DNA insertions. In the case of the A90 line, T-DNA was inserted into the At2g07682 gene, which was originated from the transferred mitochondrial DNA. On the other hand, in the case of the A96 line, we found that the T-DNA was inserted into the At5g39500 gene. Currently, we are checking whether the activation-tagged lines truly have more tolerant to submergence stress.