Identification of genes conferring tolerance to submergence or waterlogging stress and generation of submergence-tolerant crops

鉴定赋予对淹没或涝胁迫耐受性的基因并产生耐淹没作物

• 批准号: 16380004
• 负责人: NAKAZONO Mikio
• 金额: $ 10.11万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380004/
• 关键词: crop; submergence tolerance; waterlogging tolerance; rice; Arabidopsis; activation tagging; アクチベーションタギング

Rapid environmental changes subject plants to various abiotic stresses that adversely affect their growth and development. In Japan, crops (e.g., soybean, barley and wheat) are often deprived of oxygen by floods and heavy rainfall in rainy season. Because plants are sessile, their ability to respond quickly to the waterlogged or submergence stress is crucial for adaptation arid survival. To identify genes that can confer waterlogging or submergence tolerance to crops, in this project, we screened the mutants that show more tolerance to submergence using the activation-tagged mutant library and the EMS-mutagenized mutant library of Arabidopsis thaliana. Finally, we selected two mutant lines from 310,000 activation tagged lines and determined the nucleotide sequences at the border of T-DNA insertions. In the case of the A90 line, T-DNA was inserted into the At2g07682 gene, which was originated from the transferred mitochondrial DNA. On the other hand, in the case of the A96 line, we found that the T-DNA was inserted into the At5g39500 gene. Currently, we are checking whether the activation-tagged lines truly have more tolerant to submergence stress.

快速的环境变化使植物遭受各种非生物胁迫，对其生长和发育产生不利影响。在日本，农作物（例如大豆、大麦和小麦）经常因雨季的洪水和强降雨而缺氧。由于植物是无柄的，它们对淹水或淹没胁迫的快速反应能力对于适应和生存至关重要。为了鉴定能够赋予作物耐涝或耐淹性的基因，在本项目中，我们利用拟南芥激活标记突变体库和EMS诱变突变体库筛选了具有更强耐淹性的突变体。最后，我们从 310,000 个激活标记品系中选择了两个突变品系，并确定了 T-DNA 插入边界的核苷酸序列。在A90系的情况下，T-DNA被插入At2g07682基因中，该基因源自转移的线粒体DNA。另一方面，在A96系的情况下，我们发现T-DNA被插入到At5g39500基因中。目前，我们正在检查激活标记的品系是否确实具有更强的浸没压力耐受性。

项目成果

A point mutation of Adhl gene is involoved in repression of coleoptile elongation under submergence in rice.

Adhl 基因的点突变与水稻浸水下胚芽鞘伸长的抑制有关。

• 发表时间: 2006
• 期刊: Breed. Sci. 56
• 作者: Saika;et al.
• 通讯作者: et al.

cDNA microarray analysis of gene expression changes during pollination, pollen-tube elongation, fertilization, and early embryogenesis in rice pistils.

水稻雌蕊授粉、花粉管伸长、受精和早期胚胎发生过程中基因表达变化的 cDNA 微阵列分析。

• 发表时间: 2005
• 期刊: Sex. Plant Rep. 17
• 作者: Yoshida;K. T.;et al.
• 通讯作者: et al.

The rice pyruvate decarboxylase 3 gene, which lacks introns, is transcribed in mature pollen

• DOI: 10.1093/jxb/erh029
• 发表时间: 2004-01-01
• 期刊: JOURNAL OF EXPERIMENTAL BOTANY
• 影响因子: 6.9
• 作者: Li, YH;Ohtsu, K;Nakazono, M
• 通讯作者: Nakazono, M

Analysis of expression of genes for mitochondrial aldehyde dehydrogenase in maize during submergence and following re-aeration.

玉米在淹没期间和重新通气后线粒体醛脱氢酶基因表达的分析。

• 发表时间: 2006
• 期刊: BREEDING SCIENCE 56
• 作者: Meguro;N.
• 通讯作者: N.

Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection

• DOI: 10.1016/j.plantsci.2005.08.021
• 发表时间: 2006-01-01
• 期刊: PLANT SCIENCE
• 影响因子: 5.2
• 作者: Nakada, M;Komatsu, M;Kanno, A
• 通讯作者: Kanno, A