Development of genetic transformation of Japanese pear by inhibition of calcium ion influx

点击翻译按钮获取中文摘要

High rates of regeneration of adventitious shoots were obtained from the cotyledons of Japanese pears Imamuraaki and Agenosho Shinanashi from 16 Japanese pear cultivars, when the media contained 5 uM NAA combined with 10 or 25 uM BA were used. Cotyledons of Japanese pear cultivars were co-cultivated with Agrobacterium tumefaciens which contained a green fluorescent protein (GFP) gene. EGTA (a chelator of calcium) treatments and sonication were applied, which could prevent plant defense reaction and produce physical wounds across the tissue respectively. EGTA treatment did not show a positive effect on expression of GFP fluorescence whereas sonication significantly increased. One plant regenerated from Agenosho Shinanashi showed stable GFP fluorescence and confirmed as a transformant. Three other transformed regenerated shoots by myb gene showed red color, which were derived from Imamuraaki in another experiment. Transformation system in our study was shown to be reproducible.