Physiological and Biochemical Studies of Polyamines which are covalently linked to Peptidoglycan in Anaerobic Bacteria.

腐胺和尸胺现已被证明仅掺入V. alcalescens ATCC 17745的肽聚糖层中。小弧菌GAI 0574也证明含有腐胺作为肽聚糖的组分。两种韦荣球菌属物种共有的肽聚糖的主要化学结构是GlcNAc-MurNAc-L-Ala-D-Glu-meso-DAP-D-Ala。腐胺或尸胺与肽聚糖的D-谷氨酸残基的-羧基共价连接。在V. alcalescens ATCC 17745中，尸胺对肽聚糖的D-谷氨酸残基的-羧基的饱和度超过40%是正常细胞生长所必需的。利用一系列具有NH_3^+（CH_2）NH_3^+一般结构的二胺来研究这些细菌正常细胞生长所需二胺的结构特异性。结合到肽聚糖中的二氨基己烷（n=6）与腐胺（n=4）和尸胺（n=5）对于正常细胞生长一样有效。然而，二氨基丙烷（n=3）和二氨基庚烷（n=7）对于生长的效果不如二氨基己烷，尽管它们以相同的程度掺入肽聚糖中。

Both putrescine and cadaverine have now been demonstrated to be incorporated exclusively into the peptidoglycan layer of V. alcalescens ATCC 17745. V. parvula GAI 0574 also proved to contain putrescine as a component of the peptidoglycan. The primary chemical structure of the peptidoglycan common of the two Veillonella species is GIcNAc-MurNAc-L-Ala-D-Glu-meso-DAP-D-Ala. Putrescine or cadaverine links covalently to the -carboxyl group of the D-glutamic acid residue of the peptidoglycan. In V. alcalescens ATCC 17745, above 40% saturation of cadaverine to the -carboxyl group of the D-glutamic acid residue of the peptidoglycan is necessary for normal cell growth. The structural specificity of the diamine requirement for normal cell growth of these bacteria was examined by using a series of diamines with a general structure of NH_3^+(CH_2)NH_3^+. Diaminohexane (n=6) which was incorporated into the peptidoglycan was as effective as putrescine (n=4) and cadaverine (n=5) for normal cell growth. However, diaminopropane (n=3) and diaminoheptane (n=7) were less effective for growth than diaminohexane, although they were incorporated into the peptidoglycan to the same extent.