Physiological and Biochemical Studies of Polyamines which are covalently linked to Peptidoglycan in Anaerobic Bacteria.

腐胺和尸胺现在已经被证明可以完全结合到V. alcalescens ATCC 17745的肽聚糖层中。细小弧菌GAI 0574也被证明含有腐胺作为肽聚糖的一种成分。两种细孔菌共有的肽聚糖的主要化学结构是gicnac - murnac - l - ala - d - glue -meso- dap - d - ala。腐胺或尸胺共价连接到肽聚糖的d -谷氨酸残基的-羧基。在V. alcalescens ATCC 17745中，超过40%的尸胺饱和到肽聚糖的d -谷氨酸残基的-羧基是正常细胞生长所必需的。用一系列一般结构为NH_3^+(CH_2)NH_3^+的二胺，考察了这些细菌正常细胞生长所需二胺的结构特异性。二氨基己烷（n=6）掺入肽聚糖中，与腐胺（n=4）和尸胺（n=5）对正常细胞生长的影响相同。然而，二氨基丙烷（n=3）和二氨基庚烷（n=7）对生长的影响不如二氨基己烷，尽管它们在肽聚糖中的掺入程度相同。

Both putrescine and cadaverine have now been demonstrated to be incorporated exclusively into the peptidoglycan layer of V. alcalescens ATCC 17745. V. parvula GAI 0574 also proved to contain putrescine as a component of the peptidoglycan. The primary chemical structure of the peptidoglycan common of the two Veillonella species is GIcNAc-MurNAc-L-Ala-D-Glu-meso-DAP-D-Ala. Putrescine or cadaverine links covalently to the -carboxyl group of the D-glutamic acid residue of the peptidoglycan. In V. alcalescens ATCC 17745, above 40% saturation of cadaverine to the -carboxyl group of the D-glutamic acid residue of the peptidoglycan is necessary for normal cell growth. The structural specificity of the diamine requirement for normal cell growth of these bacteria was examined by using a series of diamines with a general structure of NH_3^+(CH_2)NH_3^+. Diaminohexane (n=6) which was incorporated into the peptidoglycan was as effective as putrescine (n=4) and cadaverine (n=5) for normal cell growth. However, diaminopropane (n=3) and diaminoheptane (n=7) were less effective for growth than diaminohexane, although they were incorporated into the peptidoglycan to the same extent.