Physiological and Biochemical Studies of Polyamines which are covalently linked to Peptidoglycan in Anaerobic Bacteria.

腐胺和身体碱现在已经被证明只被结合到碱性弧菌ATCC 17745的肽聚糖层中。微小弧菌GAI 0574也被证明含有腐胺作为肽聚糖的一种成分。两种韦洛氏菌共有的肽聚糖的一级化学结构为GIcNAc-MurNAc-L-丙氨酸-D-谷氨酸-Meso-DAP-D-丙氨酸。腐胺或身体碱共价连接到肽聚糖的D-谷氨酸残基的-羧基上。在碱性弧菌ATCC 17745中，身体碱对肽聚糖的D-谷氨酸残基的-羧基的饱和度超过40%是细胞正常生长所必需的。用一系列一般结构为NH_3~+(CH_2)NH_3~+的二胺研究了这些细菌正常细胞生长所需的二胺的结构特异性。氨基己烷(n=6)与腐胺(n=4)和身体碱(n=5)对细胞的正常生长同样有效。然而，二氨基丙烷(n=3)和二氨基庚烷(n=7)的生长效果不如二氨基己烷，尽管它们被结合到肽聚糖中的程度相同。

Both putrescine and cadaverine have now been demonstrated to be incorporated exclusively into the peptidoglycan layer of V. alcalescens ATCC 17745. V. parvula GAI 0574 also proved to contain putrescine as a component of the peptidoglycan. The primary chemical structure of the peptidoglycan common of the two Veillonella species is GIcNAc-MurNAc-L-Ala-D-Glu-meso-DAP-D-Ala. Putrescine or cadaverine links covalently to the -carboxyl group of the D-glutamic acid residue of the peptidoglycan. In V. alcalescens ATCC 17745, above 40% saturation of cadaverine to the -carboxyl group of the D-glutamic acid residue of the peptidoglycan is necessary for normal cell growth. The structural specificity of the diamine requirement for normal cell growth of these bacteria was examined by using a series of diamines with a general structure of NH_3^+(CH_2)NH_3^+. Diaminohexane (n=6) which was incorporated into the peptidoglycan was as effective as putrescine (n=4) and cadaverine (n=5) for normal cell growth. However, diaminopropane (n=3) and diaminoheptane (n=7) were less effective for growth than diaminohexane, although they were incorporated into the peptidoglycan to the same extent.