Construction of new breeding methods used the control of matylating pattern

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DNA methylation is one of the most important mechanisms for the control of gene expression. This pattern is changed by environment, such as stress and food, and changed pattern is transferred to next generation. Therefore, the control of methylation pattern is expected as new breeding method which is not accompanied by change of DNA sequence. In this project, the correlation between methylation patterns and several traits, such as growth, thermal tolerance and behavior, were examined in the guppy. In the case of Growth hormone gene, the methylation pattern of 5' upstream region were compared, however, clear correlation was not observed between methylation pattern and body size. In the case of thermal tolerance and behavior, correlations were not observed. On the other hand, non-lethal heat treatment leads thermal tolerance at lethal temperature was decided. It is promising as the new method to produce thermal tolerant individual. The method to measure the behavior was constructed.