Dynamics of oocyte nucleoli in mammals

哺乳动物卵母细胞核仁的动态

• 批准号: 23658226
• 负责人: MIYANO Takashi
• 金额: $ 2.5万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658226/
• 关键词: 卵母細胞; 核小体; 体外培養; プロテアソーム

During the growth stage, oocyte nucleoli are intensively engaged in ribosomal RNA (rRNA) synthesis. As the oocytes approach the finalsize, synthetic activity of RNAs decreases, and coincidently nucleoli change the morphology and reduce the volume. Nucleoliof growing oocytes werecompacted and reduced the sizeby transcription inhibitors (actinomycin D or α-amanitin). On the other hand, the nucleolus volume of oocytes significantly increased by thetreatment with proteasome inhibitors, MG-132 or lactacystin. During oocyte maturation, the nucleolus was disassembled into the cytoplasm. When the oocytes were arrested at the germinal vesicle stage, and the nucleolus was aspirated from the nucleus and transferred to the cytoplasm, the nucleolus disappeared and later it was reassembled in the nucleus.

在生长阶段，卵母细胞核仁集中参与核糖体RNA （rRNA）的合成。当卵母细胞接近最终大小时，rna的合成活性降低，核仁相应地改变形态并减小体积。转录抑制剂（放线菌素D或α-amanitin）使生长中的卵母细胞核仁压缩并减小。另一方面，用蛋白酶体抑制剂MG-132或乳糜菌素处理卵母细胞的核仁体积显著增加。在卵母细胞成熟过程中，核仁被分解成细胞质。卵母细胞在生发囊泡期被截留，核仁被从细胞核中抽离并转移到细胞质中，核仁消失后在细胞核中重新组装。

项目成果

Growing-oocyte nucleolus has the ability to inhibit meiotic progression in pig oocytes

生长卵母细胞核仁具有抑制猪卵母细胞减数分裂进程的能力

• 发表时间: 2011
• 作者: Kyogoku;H.;Ogushi;S.;Miyano;T.;Fulka;J.Jr.
• 通讯作者: J.Jr.

Nucleolus exchange between 2-cell embryos and germinal vesicle oocytes

2细胞胚胎和生发囊卵母细胞之间的核仁交换

• 发表时间: 2012
• 作者: Kyogoku;H.;Miyano;T.
• 通讯作者: T.

2細胞期胚の核小体に置換したブタ卵母細胞の発生

2 细胞期胚胎中核仁替换的猪卵母细胞的发育

• 发表时间: 2012
• 作者: 京極博久;大串素雅子;宮野隆,
• 通讯作者: 宮野隆,

ブタ卵母細胞の成熟過程における核小体の直径の変化

猪卵母细胞成熟过程中核仁直径的变化

• 发表时间: 2012
• 作者: 農端理壽;京極博久;宮野隆.
• 通讯作者: 宮野隆.

Nuclei and nucleoli in pig oocytes

猪卵母细胞的细胞核和核仁

• 发表时间: 2011
• 作者: Miyano;T.;Ogushi;S.;Kyogoku;H.;Fulka;J.Jr.
• 通讯作者: J.Jr.