权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Acquisition of maturational competence in growing oocytes correlates with their ability to active MAP kinase

生长中卵母细胞成熟能力的获得与其激活 MAP 激酶的能力相关

基本信息

批准号：
12660255
负责人：
MIYANO Takashi
金额：
$ 1.86万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660255/
关键词：
oocyte maturation MAP kinase Cdc2 kinase growth

项目摘要

Meiotic maturation of mammalian oocytes is under the control of cell cycle molecules Cdc2 kinase and MAP kinase. In the present study, I investigated the relationship between the ability to activate Cdc2 kinase and MAP kinase and the acquisition of meiotic competence during oocyte growth. In vitro growth culture of incompetent growing oocytes was also conducted to induce their maturation.1) Growing and fully grown pig oocytes were collected from antral follicles of various diameters (0.5-6.0 mm) and cultured in vitro. Fully grown oocytes (120-125 μm) which have full competence to mature to metaphase II had the ability to activate both Cdc2 and MAP kinase. In contrast, growing oocytes which have limited competence to resume meiosis had no such ability. Cyclin B1 molecules did accumulate, however, with phosphorylated inactive forms of p34^<cdc2> appearing in the cultured oocytes. Growing oocytes of 110 μm in diameter resumed meiosis but arrested at metaphase I. The oocytes were capable o … More f activating Cdc2 kinase, although they did not appear to have established a MAP kinase-activating pathway. These results suggest that pig oocytes acquire the ability to activate Cdc2 kinase and then establish the MEK-MAP-kinase pathway for full meiotic competence during the final growth phase.2) Active forms of MAP kinase (pMAPK) was localized around condensed chromosomes at metaphase I. During the progression of anaphase I and telophase I pMAPK was detected at the mid-zone of the elongated spindle. The inhibition of MAP kinase activity during the meiosis I/meiosis II transition suppressed chromosome separation, first polar body emission and formation of the metaphase II spindle. These results suggest that the spindle-associated pMAPK plays an important role in the events occurring during the meiosis I/meiosis II transition.3) Growing pig and bovine oocytes from early antral follicles grew efficiently in hypoxanthine-supplemented medium and some of them acquired the meiotic competence after 8-16 days of culture. Inhibitors of PP1/PP2A induced a rapid activation of MAP kinase in growing oocytes. Some of the treated oocytes matured to metaphase II. Less

哺乳动物卵母细胞减数分裂成熟受细胞周期分子Cdc 2激酶和MAP激酶的调控。在本研究中，我调查了激活Cdc 2激酶和MAP激酶的能力和卵母细胞生长过程中减数分裂能力的获得之间的关系。还对发育不全的卵母细胞进行体外生长培养以诱导其成熟。1）从不同直径（0.5-6.0 mm）的有腔卵泡中收集生长和完全发育的猪卵母细胞并进行体外培养。完全发育的卵母细胞（120-125 μm）具有完全成熟至中期II的能力，具有激活Cdc 2和MAP激酶的能力。相反，生长中的卵母细胞恢复减数分裂的能力有限，没有这种能力。然而，细胞周期蛋白B1分子确实积累，在培养的卵母细胞中出现磷酸化的无活性形式的p34 α<cdc2>。直径为110 μm的卵母细胞开始减数分裂，但在中期Ⅰ停止。卵母细胞能够 ...更多信息 f激活Cdc 2激酶，尽管它们似乎没有建立MAP激酶激活途径。这些结果表明，猪卵母细胞在终末发育阶段获得了激活Cdc 2激酶的能力，并建立了MEK-MAP-kinase通路，以获得完整的减数分裂能力。在后期I和末期I的进展过程中，pMAPK在细长纺锤体的中间区被检测到。在减数分裂I/减数分裂II转换期间抑制MAP激酶活性抑制染色体分离、第一极体发射和中期II纺锤体的形成。这些结果表明，纺锤体相关的pMAPK在减数分裂I/II转换过程中起重要作用。3）猪和牛早期有腔卵泡卵母细胞在添加次黄嘌呤的培养液中生长良好，部分卵母细胞在培养8-16 d后获得减数分裂能力。PP 1/PP 2A的抑制剂诱导生长中的卵母细胞中MAP激酶的快速激活。一些处理的卵母细胞成熟到中期II。少