Dissection ofvarious ovarian functions, and extraction and reconstruction of oogenesis-specific functions to develop oocyte growth culture systems for domestic animals

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Bovine and porcine primordial follicles did not develop beyond the primary stage in culture. Bovine secondary follicles develop in the bovine plasma-supplemented medium, and the oocytes (approximately 60 μm in diameter) grew to 90 μm or more after 4 weeks. The medium containing a high concentration of polyvinylpyrrolidone, estradiol-17β and androstenedione supported the growth and acquisition of meiotic competence of bovine growing oocytes (90-100 μm in diameter) isolated from early antral follicles. Porcine growing oocytes from early antral follicles grew to the final size and acquired the meiotic competence in the medium containing FSH, dbcAMP and estradiol-17β.