Analysis of the effect of environmental factors on the structure of catalytic amyloid fibrils

淀粉样原纤维是一种丝状多肽聚集体，与阿尔茨海默病、帕金森病和系统性淀粉样变性等疾病有关，具有重要的科学意义。虽然这些错误折叠状态在人体内的异常形成可能导致严重的健康问题，但由于淀粉样蛋白结构的独特结构和化学特性，越来越多的人试图将其用于生物技术目的。然而，它们的结构和对环境压力的适应能力尚不清楚。本项目旨在以催化淀粉样蛋白为模型系统，确定环境因素对淀粉样蛋白多态性结构的影响。我们将结构生物学的方法与数学方法相结合，在单颗粒水平上对纤维样品进行统计评估，并从总体上改进其结构分析。

Amyloid fibrils are filamentous polypeptide aggregates of great scientific relevance due to their association with diseases including Alzheimer’s, Parkinson’s and systemic amyloidosis. While the abnormal formation of these misfolded states inside the human body can lead to severe health problems, there are increasing attempts to utilize amyloid structures due to their unique structural and chemical properties for biotechnological purposes. However, their structure and resilience to environmental stress are not well understood. The aim of this project to use catalytic amyloid as a model system to determine the effect of environmental factors on the polymorphic structure of amyloids. We will combine methods of structural biology with mathematical approaches to statistically assess fibril samples at a single particle level and to generally improve their structural analysis.