Analysis of the effect of environmental factors on the structure of catalytic amyloid fibrils

环境因素对催化淀粉样原纤维结构的影响分析

• 批准号: 401119613
• 负责人: Professor Dr. Marcus Fändrich
• 金额: --
• 依托单位: Institut für Stochastik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/401119613?language=en
• 关键词: Analysis; effect; environmental; factors; structure

Amyloid fibrils are filamentous polypeptide aggregates of great scientific relevance due to their association with diseases including Alzheimer’s, Parkinson’s and systemic amyloidosis. While the abnormal formation of these misfolded states inside the human body can lead to severe health problems, there are increasing attempts to utilize amyloid structures due to their unique structural and chemical properties for biotechnological purposes. However, their structure and resilience to environmental stress are not well understood. The aim of this project to use catalytic amyloid as a model system to determine the effect of environmental factors on the polymorphic structure of amyloids. We will combine methods of structural biology with mathematical approaches to statistically assess fibril samples at a single particle level and to generally improve their structural analysis.

淀粉样蛋白原纤维是丝状多肽骨料，这是由于它们与包括阿尔茨海默氏症，帕金森氏症和系统性淀粉样变性在内的疾病的相关性。尽管这些不折叠状态在人体内部的异常形成可能导致严重的健康问题，但由于其独特的结构和化学特性来实现生物技术目的，因此越来越多地利用淀粉样蛋白结构的尝试。但是，它们对环境压力的结构和韧性尚不清楚。该项目使用催化淀粉样蛋白作为模型系统的目的，以确定环境因素对淀粉样蛋白多态性结构的影响。我们将结合结构生物学方法与数学方法相结合，以单个粒子水平评估原纤维样品并通常改善其结构分析。