Analysis of the effect of environmental factors on the structure of catalytic amyloid fibrils

环境因素对催化淀粉样原纤维结构的影响分析

• 批准号: 401119613
• 负责人: Professor Dr. Marcus Fändrich
• 金额: --
• 依托单位: Institut für Stochastik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/401119613?language=en
• 关键词: Analysis; effect; environmental; factors; structure

Amyloid fibrils are filamentous polypeptide aggregates of great scientific relevance due to their association with diseases including Alzheimer’s, Parkinson’s and systemic amyloidosis. While the abnormal formation of these misfolded states inside the human body can lead to severe health problems, there are increasing attempts to utilize amyloid structures due to their unique structural and chemical properties for biotechnological purposes. However, their structure and resilience to environmental stress are not well understood. The aim of this project to use catalytic amyloid as a model system to determine the effect of environmental factors on the polymorphic structure of amyloids. We will combine methods of structural biology with mathematical approaches to statistically assess fibril samples at a single particle level and to generally improve their structural analysis.

淀粉样原纤维是具有很大科学相关性的丝状多肽聚集体，这是由于它们与包括阿尔茨海默病、帕金森病和系统性淀粉样变性的疾病相关。虽然人体内这些错误折叠状态的异常形成可能导致严重的健康问题，但由于淀粉样蛋白结构独特的结构和化学性质，越来越多的人试图将其用于生物技术目的。然而，它们的结构和对环境压力的适应能力还不很清楚。本项目的目的是利用催化淀粉样蛋白作为模型系统，以确定环境因素对淀粉样蛋白多态性结构的影响。我们将结合结构生物学的联合收割机方法和数学方法，在单颗粒水平上对原纤维样品进行统计评估，并普遍改善其结构分析。