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Development of multiwavelength detection high performance liquid chromatography system for diagnosis of diseases

疾病诊断多波长检测高效液相色谱系统的研制

基本信息

批准号：
58870125
负责人：
FUNAKUBO Hiroyasu
金额：
$ 12.8万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1983
资助国家：
日本
起止时间：
1983 至 1985
项目状态：
已结题

项目摘要

In this research, an HPLC multiwavelength detection system was developed which can measure the absorption spectra of components in addition to their retention time. The developed system was also applied to actual analysis of biological fluids such as human urine to evaluate its effectiveness.Hardware controlled by several microcomputers and needed control software for the HPLC multiwavelength detection system were developed. It can measure and record spectra over a range of 200nm to 800nm. A data processing system was specially designed for effective and efficient processing of the large amounts of data obtained by multiwavelength detection. Data processing programs were developed which can extract useful information from data obtained by a single analysis of a sample. Examples of these programs are 3-dimensional displays of chromatograms and comparison of chromatograms of different samples using computer graphics. Human urine samples from patients with various diseasis and from healthy controls were analyzed. The results show that some peaks, which may appear to represent a singele component by using a monowavelength absorbance detector, are found to actually represent several components when they are analyzed using this multiwavelength detection system. In the 3D chromatograms of urine samples from some diseased groups, there are some peak patterns which are not observed in the chromatograms of urine samples from healthy groups. These results show the effectiveness of the developed system in the field of biological fluid analysis. Statistical analysis and multivariate analysis of peak heights in the chromatograms were tested to develop methods to detect these differences by automatic computer analysis. The results show that the patterns of amount changes of several components in urine samples are different between diseased groups and healthy groups. This point must be furhter investigated in future research.

本研究建立了一种高效液相色谱多波长检测系统，该系统可以测量成分的吸收光谱和保留时间。该系统还应用于实际的生物流体分析，如人体尿液，以评估其有效性。开发了HPLC多波长检测系统的硬件控制和所需的控制软件。它可以测量和记录200nm到800nm范围内的光谱。为了对多波长探测获得的大量数据进行有效、高效的处理，专门设计了一套数据处理系统。开发了数据处理程序，可以从样品的单次分析获得的数据中提取有用的信息。这些程序的例子是色谱图的三维显示和使用计算机图形对不同样品的色谱图进行比较。对不同疾病患者和健康对照者的尿液样本进行了分析。结果表明，使用单波长吸光度检测器分析时，发现一些峰可能代表单一成分，但使用多波长检测系统分析时，发现它们实际上代表多个成分。在某些疾病组尿液样品的三维色谱图中，存在一些健康组尿液样品色谱中没有观察到的峰型。这些结果表明了所开发的系统在生物流体分析领域的有效性。通过对色谱峰高的统计分析和多变量分析，建立了用计算机自动分析来检测这些差异的方法。结果表明，疾病组和健康组尿液样品中几种成分的量变化模式不同。这一点需要在今后的研究中进一步探讨。