Genetic code expansion tools to introduce post-translational modifications (C02)

引入翻译后修饰的遗传密码扩展工具（C02）

• 批准号: 401883760
• 金额: --
• 依托单位: Laboratorium für Organische Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/401883760?language=en
• 关键词: Genetic; code; expansion; tools; introduce

Protein post-translational modifications (PTMs) are important for regulating diverse aspects of cellular physiology. Within this CRC we want to leverage genetic code expansion approaches - in conjunction with in vivo chemistries and enzymatic reactions – to site-specifically incorporate PTMs such as succinylation and ubiquitination into proteins in vivo. Such approaches will help us deciphering their roles – in collaboration with other groups of this CRC and in the Munich area – in nucleosome dynamics, DNA replication and transcription and protein localization. Furthermore these tools will benefit from the advantage of being generally applicable to diverse biological questions.

蛋白质翻译后修饰（PTMs）对于调节细胞生理学的各个方面是重要的。在该CRC中，我们希望利用遗传密码扩增方法-结合体内化学和酶促反应-将PTM如琥珀酰化和泛素化位点特异性地掺入体内蛋白质中。这些方法将帮助我们破译他们的作用-与其他小组的CRC和在慕尼黑地区-在核小体动力学，DNA复制和转录和蛋白质定位。此外，这些工具将受益于普遍适用于各种生物学问题的优势。