Genetic code expansion tools to introduce post-translational modifications (C02)

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Protein post-translational modifications (PTMs) are important for regulating diverse aspects of cellular physiology. Within this CRC we want to leverage genetic code expansion approaches - in conjunction with in vivo chemistries and enzymatic reactions – to site-specifically incorporate PTMs such as succinylation and ubiquitination into proteins in vivo. Such approaches will help us deciphering their roles – in collaboration with other groups of this CRC and in the Munich area – in nucleosome dynamics, DNA replication and transcription and protein localization. Furthermore these tools will benefit from the advantage of being generally applicable to diverse biological questions.