Studies on the identification of ABH antigens between red cell and saliva

红细胞与唾液ABH抗原鉴定的研究

• 批准号: 59870023
• 负责人: SAGISAKA Kaoru
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59870023/
• 关键词: ABH blood group; Red cell; Saliva

To distinguish ABH antigens between red cell and saliva, we attempted to immunize various animals with red cell and saliva. Absorption of rabbit antisera to O . A red cell, O. B saliva with appropriate red cell and saliva resulted in specific antibody to the immunogen (anti-Hr,-Ar,-Hs and Bs). Rabbit was divided into two groups ( <A^+> and <A^-> ) according to its secreting ability of A antigen in saliva. Specific anti-Ar was produced in only <A^+> rabbit. It meant that A+ rabbit had A antigen shared with human A saliva. On the other hand, anti-As could be prepared in rabbit but not in guinea pig. The agglutinin titers of anti-Hr,-Hs and Bs) were relatively high, but the others were low. The titers of the antisera to saliva antigen were tested with latex coated with saliva of the corresponding group. However, anti-Br was raised in neither rabbit, guinea pig nor chicken. In this experiment, antiHr,Hs,Bs sera were easily prepared but antiAr and As were produced after immunization of many trials of immunization. At any rate, it was considered that these antisera specific for red cell or saliva ABH antigens were useful for forensic practice in criminal cases.

为了区分红细胞和唾液中的ABH抗原，我们尝试用红细胞和唾液免疫各种动物。兔抗O .红细胞O. B唾液与适当的红细胞和唾液产生特异性抗体的免疫原（抗-Hr，-Ar，-Hs和Bs）。根据家兔唾液分泌A抗原的能力将其分为<A^+>和<A^->两组。仅<A^+>兔产生特异性抗Ar。说明A+兔具有与人A唾液相同的A抗原。另一方面，在家兔体内可制备抗-As，而在豚鼠体内则不能。抗-Hr、-Hs和Bs的凝集素效价较高，而其它的凝集素效价较低。用相应组的唾液包被胶乳检测抗唾液抗原的抗血清滴度。但兔、豚鼠和鸡均未产生抗Br抗体。本实验中抗Hr、Hs、Bs血清制备容易，而抗Ar、As血清需经多次免疫试验才能产生。无论如何，认为这些红细胞或唾液ABH抗原特异性抗血清在刑事案件的法医实践中是有用的。

项目成果

Oral presentation at 69th annual meeting of Medico-Legal Society of Japan held at Morioka on 22-24 May, 1985. (1985)

1985 年 5 月 22-24 日在盛冈举行的日本法医学会第 69 届年会上的口头报告。(1985)

日本法医学雑誌. 39巻-6号. (1985)

日本法医学杂志。第 39 卷第 6 期（1985 年）