Physico-chemical stability of blood group-specific immunoglobulins in the state of immunocomplex

免疫凝集素或沉淀素通过加热或某些化学物质失去与相应抗原的反应性。分别用人IgM抗a和兔IgG抗M血清致敏se组和M组血清致敏。用生理盐水洗涤后，加热或用福尔马林或2-巯基乙醇（2-ME）或不同浓度处理。通过混合凝集或洗脱试验检测免疫复合物状态下抗体的免疫反应性，表明免疫复合物状态下的IgM和IgG抗体对150℃加热10 min和高浓度的福尔马林和2-ME具有抵抗性。认为IgM和IgG虽然本质上是耐热性和化学性的，但通过形成免疫复合物获得了一种新的耐热性和化学稳定性

Immune agglutinin or precipitin loses its reactivity with the corresponding antigen by heating or some chemicals. A.Se group seliva or M group blood stain was sensitized with human IgM anti-A or rabbit IgG anti-M serum,respectively. After being washed with saline, the stains were heated or treated with formalin or 2-mercaptoethanol(2-ME) or various concentrations. The immunoreactivity of the antibodies in the state of immunecomplex was examined by the mixed agglutination or elution test, indicating IgM and IgG antibodies in the state of immunecomplex were proof against heating up to 150C for 10 min and against formalin and 2-ME of high concentrations. It is considered that IgM and IgG acguire a new property of heat and chemical stability by formation of immunecomplex although they are substantially heat and chemical fabile