Physico-chemical stability of blood group-specific immunoglobulins in the state of immunocomplex

免疫复合物状态下血型特异性免疫球蛋白的理化稳定性

• 批准号: 61570285
• 负责人: SAGISAKA Kaoru
• 金额: $ 1.22万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570285/
• 关键词: immunecomplex; bood group; 血液型; 耐熱性; メルカプトエタノール; 免疫複合体; 熱耐性

Immune agglutinin or precipitin loses its reactivity with the corresponding antigen by heating or some chemicals. A.Se group seliva or M group blood stain was sensitized with human IgM anti-A or rabbit IgG anti-M serum,respectively. After being washed with saline, the stains were heated or treated with formalin or 2-mercaptoethanol(2-ME) or various concentrations. The immunoreactivity of the antibodies in the state of immunecomplex was examined by the mixed agglutination or elution test, indicating IgM and IgG antibodies in the state of immunecomplex were proof against heating up to 150C for 10 min and against formalin and 2-ME of high concentrations. It is considered that IgM and IgG acguire a new property of heat and chemical stability by formation of immunecomplex although they are substantially heat and chemical fabile

免疫凝集素或沉淀素因加热或某些化学物质而失去与相应抗原的反应性。 A、Se组唾液或M组血染分别用人IgM抗A或兔IgG抗M血清致敏。用盐水洗涤后，将污渍加热或用福尔马林或2-巯基乙醇(2-ME)或各种浓度处理。通过混合凝集或洗脱试验检测免疫复合物状态的抗体的免疫反应性，表明免疫复合物状态的IgM和IgG抗体能够抵抗150℃加热10分钟以及抵抗高浓度的福尔马林和2-ME。尽管 IgM 和 IgG 本质上对热和化学不稳定，但认为它们通过形成免疫复合物而获得了热和化学稳定性的新性质。

项目成果

Aoki,Sagisaka et al.: Act.Crim.Jpn.52. 159-162 (1986)

青木、鹭坂等人：Act.Crim.Jpn.52。

Sagisaka,funayama et al.: Act.Crim.Jpn.52. 225-228 (1986)

Sagisaka,funayama 等人：Act.Crim.Jpn.52。

Funayama,Sagisaka et al.: Tohoku J.exp.Med.149. 407-416 (1986)

Funayama，Sagisaka 等人：Tohoku J.exp.Med.149。

Ohya,SagiSaka et al.: Tohoku J.exp.Med.150. 309-315 (1986)

Ohya，SagiSaka 等人：Tohoku J.exp.Med.150。

Kojima,Sagisaka et al.: Forensic Sci.Int.32. 67-77 (1986)

小岛、鹭坂等人：《法医学国际》32。