Application of DNA analyses with single locus probes to personal identification and paternity cases

点击翻译按钮获取中文摘要

To improve the accuracy of personal identification and paternity cases in forensic practice, we have examined seven kinds of DNA probes recognizing hypervariable DNA loci. The combinations of probe and restriction enzyme were as follows ; MR24/1-Hinf l, 3 ' Globin-PvnII, Ii a- ras-PvnII, Mucin-PvnII, D2S44(pY NH 24)lYspl, D17S30(PYNZ22)-Ilspl, DlS57(PYNZ2)-Rsal. The polymorphic alleles were 37, 39, 5, 10, 33, 15, and 5, respectively. Exclusion probability(EP)and paternity index(PI)were caluculated from the allelic frequency and its distributions among Japanese population. Cumulative EP from 7 DNA probes was 0.9999 32, and cumulative PI ranged from 7.3 X 10^6 to 770. Also, cumulative EP from 17 kinds of conventional blood group markers(CBGM)was 0.9776, and cumulative PI ranged from 1290 to 0.11. Total EP from 7 DNA probes and CBGM was 0.9999984 78. Cumulative PI from 7 DNA probes were 4.5 to 2, 000, 000 times higher than that from CBGM. Therefore, the single locus hypervariable DNA polymorphisms are considered to be informative for personal identification and paternity test.