Application of DNA analyses with single locus probes to personal identification and paternity cases

单基因座探针 DNA 分析在个人鉴定和亲子鉴定案件中的应用

• 批准号: 02670252
• 负责人: SAGISAKA Kaoru
• 金额: $ 1.47万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670252/
• 关键词: Personal identification; Paternity test; Single locus DNA probe; VNTR; HVR; Apo B gene; PCR; ApoB遺伝子

To improve the accuracy of personal identification and paternity cases in forensic practice, we have examined seven kinds of DNA probes recognizing hypervariable DNA loci. The combinations of probe and restriction enzyme were as follows ; MR24/1-Hinf l, 3 ' Globin-PvnII, Ii a- ras-PvnII, Mucin-PvnII, D2S44(pY NH 24)lYspl, D17S30(PYNZ22)-Ilspl, DlS57(PYNZ2)-Rsal. The polymorphic alleles were 37, 39, 5, 10, 33, 15, and 5, respectively. Exclusion probability(EP)and paternity index(PI)were caluculated from the allelic frequency and its distributions among Japanese population. Cumulative EP from 7 DNA probes was 0.9999 32, and cumulative PI ranged from 7.3 X 10^6 to 770. Also, cumulative EP from 17 kinds of conventional blood group markers(CBGM)was 0.9776, and cumulative PI ranged from 1290 to 0.11. Total EP from 7 DNA probes and CBGM was 0.9999984 78. Cumulative PI from 7 DNA probes were 4.5 to 2, 000, 000 times higher than that from CBGM. Therefore, the single locus hypervariable DNA polymorphisms are considered to be informative for personal identification and paternity test.

为了在法医实践中提高个人识别和亲子鉴定的准确性，我们检测了七种识别高变DNA基因座的DNA探针。探针和限制性内切酶的组合如下：MR24/1-Hinf L、3‘珠蛋白-PvnII、IIa-ras-PvnII、粘蛋白-PvnII、D2S44(Py N24)1Ysp1、D17S30(PYNZ22)-Ilsp1、DlS57(PYNZ2)-RSA1。多态等位基因分别为37、39、5、10、33、15和5。根据等位基因频率及其在日本人群中的分布，计算排除概率(EP)和亲子指数(PI)。7个DNA探针的累积等电点为0.9999 32，累积等电点为7 3×10 6~770。17种常规血型标志物的累积EP为0.9776，累积PI为1290~0.11。7个DNA探针和CBGM的总EP为0.9999984 78。7个DNA探针的累积等电点是CBGM的4.5-2000倍。因此，单基因座高变数DNA多态被认为是个体识别和亲子鉴定的有用信息。

项目成果

Tsuyoshi Yokoi: "Investigation of paternity establishing without the putative father using hypervariable DNA probes" The Japanses journal of human genetics. 35. 235-244 (1990)

Tsuyoshi Yokoi：“使用高变 DNA 探针在没有假定父亲的情况下建立亲子关系的调查”日本人类遗传学杂志。

YOKOI T, NATA M, AOKI Y and SAGISAKA K.: "An unexcluded paternity case investigated with hypervariable DNA loci." Transfusion. 30. 819-823 (1990)

YOKOI T、NATA M、AOKI Y 和 SAGISAKA K.：“利用高度可变的 DNA 基因座调查了一起未排除的亲子鉴定案例。”

那谷 雅之: "Single locus DNA probeを用いた親子鑑定" 日本法医学雑誌. 45. 138-145 (1991)

Masayuki Natani：“使用单基因座 DNA 探针进行亲子鉴定”《日本法医学杂志》45. 138-145 (1991)。