BLOOD TYPING OF MIXED-STAIN COMSISTING OF THE SAME KIND OF BODY FLUIDS

同类体液混合染色的血型分析

• 批准号: 06670448
• 负责人: SAGISAKA Kaoru
• 金额: $ 1.41万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670448/
• 关键词: MIXED STAINS; ABH BLOOD GROUP; ELISA; 混合斑痕; ABH式血液型; Mixedstain,; ABH blood group,

FOR ABH BLOODTYPING MIXED-STAIN COMSISTING OF THE SAME KIND OF BODY FLUIDS,WE TRYED TO SPECIFIC ABSORBE A OR B BLOOD EPITOPE BY NITROCELLULOSE OR MICROTITE PLATE WHICH WAS COATED WITH ANTI-A OR-B ANTIBOY.HOWEVER,WE COULD NOT ABSORBE A OR B EPITOPE DUE TO LOW CAPACITY OF THE MEMBRANE AND PLATE.TO SEPARATE ABH EPITOPES OF MIXED STAIN SCOMSISTING OF DIFFERENT KIND OF BODY FLUIDS,WETTIED TO PREPARE MONOCLONAL ANTLIBODY AGAINST TEAR OR AMNITIC FLUID.IT WAS REVEALED THAT BOTH THE ANTIBOIES HAD SPECIFICITY TO PROTEINS IN TEAR OR AMNITIC FLUID carrying ABH blood group epitopes. IT IS CONSIDERED THAT THE MONOCLONAL ANTIBOIES COULD BE APLLYED TO FORENSIC PRACTICE TO DETECT SEPARATELY ABH BLOOD GROUP FROM MIXED STAINS CONSISTING OF DIFFERENT KIND OF BODY FLUID.

为分离两种体液混合斑的ABH血型，我们尝试用包被抗A或抗B抗体的硝酸纤维素或微孔板特异性吸附A或B血型表位，但由于膜和板的容量低，不能吸附A或B血型表位。结果表明，这两种抗体对泪液或羊水中携带ABH血型表位的蛋白质具有特异性。认为该单克隆抗体可应用于不同体液混合斑中ABH血型的分离检测。

项目成果

Nagae, H., Nata, M., Aoki, Y. and Sagisaka, K.: "Preparatin of tear specific antibody and its application to foreusic pratice" Act. Crim. Jpn. 61. 181-186 (1995)

Nagae, H.、Nata, M.、Aoki, Y. 和 Sagisaka, K.：“泪液特异性抗体的制备及其在法医实践中的应用”法案。

長江美紀子,勾坂馨: "ヒト羊水に特異的なマウス車クローン抗体の作成とそれによる羊水のABH式血液型判定" 法医学の実際と研究. 37. 81-86 (1994)

Mikiko Nagae、Kaoru Kosaka：“人羊水特异性小鼠汽车克隆抗体的创建及其羊水的 ABH 血型测定”法医学实践与研究 37. 81-86 (1994)。

伊谷雅之,勾坂馨: "PCR法によるMH式血液型判定法の法医学的応用" DNA多型. 2. 135-138 (1994)

Masayuki Itani、Kaoru Kosaka：“使用 PCR 方法测定 MH 血型的方法的法医应用”DNA 多态性。 2. 135-138 (1994)。

Nagae, H., Aoki, Y., Nata, M., Hashiyada, M. and Sagisaka, K.: "Monoclonal antibody against au amuiotic protein carrying ABH blood group epitopes and its foreusic application" Tohoku J. Exp. Med.177 (in press). (1995)

Nagae, H.、Aoki, Y.、Nata, M.、Hashiyada, M. 和 Sagisaka, K.：“针对携带 ABH 血型表位的 au amuiotic 蛋白的单克隆抗体及其法医应用”Tohoku J. Exp。

伊谷雅之,勾坂馨: "血痕からのDNA抽出とPCRによる多型検出" 法医学の実際と研究. 37. 49-53 (1994)

Masayuki Itani、Kaoru Kosaka：“血迹中的 DNA 提取和 PCR 多态性检测”法医学实践与研究 37. 49-53 (1994)。