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Adhesion-Preventive Biomaterials

防粘连生物材料

基本信息

批准号：
59890009
负责人：
IKADA Yoshito
金额：
$ 5.06万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

项目摘要

Postoperative adhesion of tissues sometimes cause severe problems. Therefore, several techniques have been attempted to prevent tissue adhesion, for instance, by applying viscous aqueous solutions of polymer to protect the tissue. Our method is to insert a bioabsorbable sheet between the tissues where adhesion will possibly take place. For this purpose, a soft, flexible sheet was prepared from a copolymer of D,L-lactide and <epsilon> -caprolactone. The caprolactone monomer was copolymerized so as to reduce the softening temperature of the sheet. The biodegradation rate of the sheet is important in addition to the flexibility. L-Lactide-caprolactone copolymers of 65:35 and 88:12 (by mole) showed 100 % weight loss after 20 and 24 weeks, respectively, when immersed in PBS at 37 C. To examine the effectiveness of our bioabsorbable sheet in vivo, the pericardium and the parietal pleura of the same dog was partially ablated and the bioabsorbable sheet (D,L-lactide-caprolactone 80:20, copolymer) with a size of 4 x 8 <cm^2> was fixed in between. Inspection at 3rd month postoperation revealed no inflammation and no symptom of adhesion of the surrounding tissues. Also, a study was undertaken to examine the possibility of prevention of the scar formation after lumbar laminectomy by use of poly-D,L-lactide sheet containing elastase that would prevent the growth of collagen fibrils. Experiments on rabbits showed that the sheet containing elastase more effectively suppressed the scar formation than the sheet without elastase.

术后组织粘连有时会导致严重的问题。因此，已经尝试了多种技术来防止组织粘连，例如，通过施加聚合物的粘性水溶液来保护组织。我们的方法是在可能发生粘连的组织之间插入生物可吸收片。为此，由D，L-丙交酯和ε-己内酯的共聚物制备柔软的柔性片材。将己内酯单体共聚以降低片材的软化温度。除了柔韧性之外，片材的生物降解率也很重要。当浸入 37°C 的 PBS 中时，65:35 和 88:12（摩尔比）的 L-丙交酯-己内酯共聚物分别在 20 周和 24 周后显示出 100% 的体重减轻。为了检查我们的生物可吸收片在体内的有效性，同一只狗的心包和壁层胸膜被部分消融，生物可吸收片将尺寸为 4 x 8 <cm^2> 的（D,L-丙交酯-己内酯 80:20，共聚物）固定在其间。术后第3个月检查未见炎症，周围组织无粘连症状。此外，还进行了一项研究，探讨使用含有弹性蛋白酶的聚-D,L-丙交酯片预防腰椎椎板切除术后疤痕形成的可能性，该弹性蛋白酶可防止胶原纤维的生长。对兔子的实验表明，含有弹性蛋白酶的片材比不含弹性蛋白酶的片材更有效地抑制疤痕形成。