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Application ob hydrogen high pressure bioreactor using immobilized microorganisms

固定化微生物氢气高压生物反应器的应用

基本信息

批准号：
60850170
负责人：
NISHIMURA Shigeo
金额：
$ 4.86万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

Immobilized microorganisms have been used for NADH regeneration under hydrogen high pressure. This NADH regeneration system has been applied to production of L-phenylalanine. We attempt to produce L-phenlalanine from phenylpyruvate by immobilized cells of Nocardis opaca. N.opace was cultivated in nutrient broth. The cultured cells were immobilized in 2% Ca-clginate. Continual production of phenylalanine was carried out in the medium containing phenylpyruvate, NH_4Cl and immobilized whole cells at 37゜C for 10h under 100atm hydrogen. Production rate of phenylalanine was 0.24 umol/min-cm^3 gel with a conversion of 82%. Immobilized cells retained 76% of phenylalanine production rate after 10h reactions were repeated 11 times with two intervening reactivation.The immobilized N.opaca was applied to phenylalanine methyl ester production. NADH regeneration system was also cooperated in this system. production. of phenylalanine methyl ester was carrid out in water-organic solvent system containing phenlpyruvate methy ester, NH_4 Cl, NAD^+ and immobilized cells in the presence of hydrogen at 100atm. Immobilized cells of N. opara produced phenylalanine methy ester at the rate of 0.02 <micrn>mol/ min/cm^3 gel by 36h activated immobilized cells with a conversion of 80%. The phenylalanine methyl ester production was followed by synthesis of zaspartame from phenylpyruvate methyl ester, NH_4Cl, NAD^+, Z-aspartic acid by immobilized N.opara - thermolysin. production rate of Z-aspartame was 22.4 <micrn>mol/min/g thermolysin. The conversion was 86%.Hydrogenation of fatty acid was carried out by metal catalysts and immobilized tumen bacteria. Linoleic acid was hydrogenated with high selectivity to oleic acid by palladium catalysts in the presence og added phenylacetaldehyde. Oleic acid was hydrogenated to steatic acid by immobilized Fusocillus sp. in water-ethyl acetate system.

固定化微生物用于氢气高压下NADH的再生。该NADH再生系统已应用于L苯丙氨酸的生产。我们尝试用固定化诺卡德菌细胞由苯丙酮酸生产L-苯丙氨酸。在营养肉汤中培养诺佩斯。将培养的细胞固定在2%的碳酸钙溶液中。在含有苯丙酮酸、氯化铵和固定化全细胞、37゜℃、100大气压氢气中连续生产苯丙氨酸10h。苯丙氨酸的产率为0.24umol/min·cm~(-3)凝胶，转化率为82%。固定化细胞在两次中间再活化的情况下，重复反应10h，苯丙氨酸的产率保持在76%。该系统还配合了NADH再生系统。制作。在100 atm氢气存在下，苯丙酮酸甲酯、NH_4Cl、NAD~+和固定化细胞的水-有机溶剂体系中，苯丙氨酸甲酯被催化生成苯丙氨酸甲酯。固定化细胞经36h的活化后，以0.02微米/分/厘米~3凝胶的速率产生苯丙氨酸甲酯，转化率达80%。以苯丙酮酸甲酯、NH4Cl、NAD~+、Z-天冬氨酸为原料，固定化诺帕拉-热裂解酶合成了扎巴甜。Z-天冬氨酸氨基转移酶的产率为22.4m ol/m in/g热裂解酶。利用金属催化剂和固定化图们菌进行脂肪酸的加氢反应，转化率为86%。在添加苯乙醛的条件下，钯催化剂催化亚油酸高选择性加氢合成油酸。固定化梭状芽孢杆菌催化油酸加氢生成硬脂酸。在水-乙酸乙酯体系中。