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Development of a Specific Micro-determination Method of Gizzerosine, an Inducer of Gizzard Erosion in Chicken

鸡砂囊糜烂诱导剂 Gizzerosine 的特异性微量测定方法的建立

基本信息

批准号：
60860009
负责人：
NAITO Hiroshi
金额：
$ 5.7万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

1. A fluorometric determination method of gizzerosine was developed employing high-performance liquid chromatography. By this method, 0.2 to 5.0 g of gizzerosine in Fish meals was quantitated with the recovery of 95-102 %. fish meals, which induced severe gizzard erosion in chicks, contained more than 20 mg/kg of gizzerosine.2. Employing the newly developed determination method, the conditions of gizzerosine formation in fish meals were examined. When the pH of fish meat was slightly acidic and when the temperature during drying was more than 100゜C, significant amount of gizzerosine was formed in the fish meal.3. If lysine or ascorbic acid was included in fish meat before drying, the formation of gizzerosine was prevented. This method made it possible to produce low-gizzerosine fish meal.4. Mucosal cells of chicken proventriculus were prepared employing collagenase digestion method. Gizzerosione enhanced the oxygen uptake by these cells and the activity was inhibited by antagonists of histamine H_2-receptor. This suggests that gizzard erosion induced by gizzerosine can be prevented by cimetidine and its related compounds. This is anothor possible method to prevent gizzard erosion in chicks.5. The above-mentioned determination method was relatively complex. So, another method was tried to be developed. By using ion-exchange high performance liquid chromatography, gizzerosine was determined within 1 or 2 days. A practical procedure for wide usage of this method is now being developed.

1.建立了吉兹罗辛的高效液相色谱荧光检测方法。用该方法测定鱼粉中0.2~5.0g吉泽罗辛，加标回收率为95~102%。鱼粉中含有超过20毫克/公斤的吉兹罗辛，对雏鸡造成严重的肌胃侵蚀。采用最新建立的测定方法，考察了鱼粉中吉兹罗辛的形成条件。当鱼肉的pH稍偏酸性时，干燥过程中的温度高于100゜C时，鱼粉中会产生大量的吉兹罗辛。如果在干燥前在鱼肉中加入赖氨酸或抗坏血酸，就可以防止吉兹罗辛的形成。这种方法使生产低Gizzerosine鱼粉成为可能。采用胶原酶消化法制备鸡腺胃粘膜细胞。Gizzerosione可增加这些细胞的氧摄取，组胺H_2受体拮抗剂可抑制其活性。提示西咪替丁及其相关化合物可预防吉兹罗辛所致的肌胃糜烂。这是预防雏鸡肌胃侵蚀的一种可能的方法。上述测定方法较为复杂。因此，另一种方法被尝试开发出来。采用离子交换高效液相色谱仪，在1~2天内即可检测到吉兹罗辛。目前正在开发一种广泛使用这种方法的实用程序。