权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of a Specific Micro-determination Method of Gizzerosine, an Inducer of Gizzard Erosion in Chicken

鸡砂囊糜烂诱导剂 Gizzerosine 的特异性微量测定方法的建立

基本信息

批准号：
60860009
负责人：
NAITO Hiroshi
金额：
$ 5.7万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

1. A fluorometric determination method of gizzerosine was developed employing high-performance liquid chromatography. By this method, 0.2 to 5.0 g of gizzerosine in Fish meals was quantitated with the recovery of 95-102 %. fish meals, which induced severe gizzard erosion in chicks, contained more than 20 mg/kg of gizzerosine.2. Employing the newly developed determination method, the conditions of gizzerosine formation in fish meals were examined. When the pH of fish meat was slightly acidic and when the temperature during drying was more than 100゜C, significant amount of gizzerosine was formed in the fish meal.3. If lysine or ascorbic acid was included in fish meat before drying, the formation of gizzerosine was prevented. This method made it possible to produce low-gizzerosine fish meal.4. Mucosal cells of chicken proventriculus were prepared employing collagenase digestion method. Gizzerosione enhanced the oxygen uptake by these cells and the activity was inhibited by antagonists of histamine H_2-receptor. This suggests that gizzard erosion induced by gizzerosine can be prevented by cimetidine and its related compounds. This is anothor possible method to prevent gizzard erosion in chicks.5. The above-mentioned determination method was relatively complex. So, another method was tried to be developed. By using ion-exchange high performance liquid chromatography, gizzerosine was determined within 1 or 2 days. A practical procedure for wide usage of this method is now being developed.

1.建立了高效液相色谱荧光测定gizzerosine的方法。该方法可定量鱼粉中0.2～5.0 g的gizzerosine，回收率为95～102%。鱼粉中gizzerosine含量超过20mg/kg，可引起雏鸡严重砂囊糜烂。2．采用新开发的测定方法，检查了鱼粉中gizzerosine 的形成条件。当鱼肉的pH值呈微酸性且干燥温度超过100゜C时，鱼粉中会形成大量的gizzerosine。3．如果干燥前的鱼肉中含有赖氨酸或抗坏血酸，则可以防止gizzerosine的形成。该方法使生产低gizzerosine鱼粉成为可能。4．采用胶原酶消化法制备鸡腺胃粘膜细胞。 Gizzerosione增强了这些细胞的摄氧量，但其活性被组胺H_2受体拮抗剂抑制。这表明西咪替丁及其相关化合物可以预防由gizzerosine引起的肌胃糜烂。这是防止雏鸡砂囊糜烂的另一种可能的方法。5．上述测定方法较为复杂。因此，尝试开发另一种方法。通过使用离子交换高效液相色谱法，可在 1 或 2 天内测定 gizzerosine。目前正在开发广泛使用该方法的实用程序。