Development of a Specific Micro-determination Method of Gizzerosine, an Inducer of Gizzard Erosion in Chicken

鸡砂囊糜烂诱导剂 Gizzerosine 的特异性微量测定方法的建立

基本信息

批准号：
60860009
负责人：
NAITO Hiroshi
金额：
$ 5.7万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

1. A fluorometric determination method of gizzerosine was developed employing high-performance liquid chromatography. By this method, 0.2 to 5.0 g of gizzerosine in Fish meals was quantitated with the recovery of 95-102 %. fish meals, which induced severe gizzard erosion in chicks, contained more than 20 mg/kg of gizzerosine.2. Employing the newly developed determination method, the conditions of gizzerosine formation in fish meals were examined. When the pH of fish meat was slightly acidic and when the temperature during drying was more than 100゜C, significant amount of gizzerosine was formed in the fish meal.3. If lysine or ascorbic acid was included in fish meat before drying, the formation of gizzerosine was prevented. This method made it possible to produce low-gizzerosine fish meal.4. Mucosal cells of chicken proventriculus were prepared employing collagenase digestion method. Gizzerosione enhanced the oxygen uptake by these cells and the activity was inhibited by antagonists of histamine H_2-receptor. This suggests that gizzard erosion induced by gizzerosine can be prevented by cimetidine and its related compounds. This is anothor possible method to prevent gizzard erosion in chicks.5. The above-mentioned determination method was relatively complex. So, another method was tried to be developed. By using ion-exchange high performance liquid chromatography, gizzerosine was determined within 1 or 2 days. A practical procedure for wide usage of this method is now being developed.

1。使用高性能液相色谱法开发了一种荧光测定方法。通过这种方法，对鱼粉中的0.2至5.0 g的gizzerosine进行了定量，回收率为95-102％。鱼粉诱导了雏鸡严重的牙齿侵蚀，含有超过20 mg/kg的gizzerosine.2。采用新开发的确定方法，检查了鱼类粉中的辣椒菌素形成的条件。当鱼肉的pH值略有酸性时，温度干燥大于100°C时，在鱼粉中形成了大量的辣椒苷。3。如果在干燥前将赖氨酸或抗坏血酸包括在鱼肉中，则可以防止发作。这种方法使生产低辣酱的鱼粉成为可能。4。采用胶原酶消化方法制备了鸡胸骨植物的粘膜细胞。 izzerosion增强了这些细胞的氧气摄取，活性受到组胺H_2受体受体的拮抗剂的抑制。这表明，甲甘氨酸及其相关化合物可以预防由gizzerosine诱导的腐蚀侵蚀。这是防止小鸡侵蚀的另一种可能的方法5。上述确定方法相对复杂。因此，尝试开发另一种方法。通过使用离子 - 交换高性能液相色谱法，在1或2天内确定了izzerosine。现在正在开发用于广泛使用此方法的实际程序。