Mechanism of the effect of gizzerosine, an inducer of gizzard erosion in chicks, on acid secretion by isolated mucosal cells of chicken proventriculus.

鸡砂囊糜烂诱导剂 gizzerosine 对鸡腺胃离体粘膜细胞酸分泌的影响机制。

基本信息

批准号：
61560085
负责人：
NAITO Hiroshi
金额：
$ 1.22万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Mucosal cells of the chicken proventriculus were isolated by a collagenase perfusion method and O_2 uptake by the isolated cells was measured as an index of the activity of gastric acid secretion. Oxygen consumption was enhanced by histamine; this effect was augmented by the coexistence of isobutylmethylxanthine, an inhibitor of cyclic adenosine 5'-monophosphate (AMP) phosphodiesterase, and suppressed by imidazole, an activator of the enzyme. The action of histamine was inhibited by cimetidine, an antagonist of the histamine H_2 receptor. These results indicate that the isolated cells retained the capacity to take up O_2 responding to histamine via the H_2 receptor and probably the cyclic AMP level. Gizzerosine also stimulated O_2 consumption by the isolated cells. The effect of gizzerosine was cancelled by cimetidine, suggestig that the mechanism by which gizzerosine acts on the mucosal cells is similar to that of histamine action.Gizzerosine increased intracellular cyclic AMP level, reaching a plateau within 30 min. Similar maxima of cyclic AMP level was observed in the presence of histamine of gizzerosine. However, the potency of gizzerosine was approximately a thousand fold higher than that of histamine. The action o gizzerosine was depressed by cimetidine but not by pyrilamine, a histamine H_1-receptor antagonist, indicating that gizzerosine is a very strong histamine H_2-receptor agonist.

通过胶原酶灌注方法分离鸡肉胸骨植物的粘膜细胞，并测量分离的细胞的O_2摄取作为胃酸分泌活性的指数。组胺增强了氧气消耗。异丁基甲基黄嘌呤的共存增强，这是一种环状腺苷5'-单磷酸（AMP）磷酸二酯酶的抑制剂，并被酶的激活剂咪唑抑制。组胺H_2受体的拮抗剂Cimetidine抑制了组胺的作用。这些结果表明，孤立的细胞保留了通过H_2受体以及循环AMP水平摄取组胺的O_2的能力。 Gizzerosine还刺激了分离的细胞消耗O_2。锡辛丁取消了焦油菌的作用，暗示着焦霉素对粘膜细胞的机制与组胺作用相似。Gizzerosine提高了细胞内循环AMP水平，在30分钟内达到了高原。在存在izzerosine组胺的情况下，观察到了相似的环状AMP水平的最大值。然而，焦油的效力比组胺高约一千倍。 Cimetidine抑郁症，而不是组胺H_1受体拮抗剂吡咯胺抑郁症，这表明Gizzerosine是一种非常强大的组胺H_2受体受体激动剂。