Development of the method for continuous and in situ determination of ions in circulating blood.

开发连续原位测定循环血液中离子的方法。

基本信息

  • 批准号:
    60870007
  • 负责人:
  • 金额:
    $ 2.69万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
  • 财政年份:
    1985
  • 资助国家:
    日本
  • 起止时间:
    1985 至 1986
  • 项目状态:
    已结题

项目摘要

A new continuous measuring system for blood Na and K concentrations was developed. A small extracorporeal circulation was made and the blood was passed through Na and K sensitive glass electrodes. Applying the system to conscious rats and dogs, applicability of the method for physiological analyses was shown.Measurements of [Na] and [K] were made using Na and K sensitive glass electrodes and a reference electrode mounted on a temperature control unit of an electrolyte analyzer (SERA-230, Horiba, Kyoto). In the present system, the liquid junction used in the commercial analyzer was replaced by 3.3 mol/1 KCl agar bridge adjusting pH and Na concentration of the agar to those of blood. The accuracy of the determination was about 1.5 meq/1 of Na and less than 0.1 meq/1 of K concentration within physiological range. The effect of erythrocytes with 42 to 51% of hematocrit on reading was within 0.1 mV.Animal experiments were performed on rats and dogs, and used for the analysis of drinking behavior. The results showed that the system is sensitive enough to detect the changes in Na concentration consequent to drinking behavior and also to detect difference of salinity of drinking water. These results indicate that the present method is a potential tool for not only for physiological studies but also for clinical use in the fields of nephrology and cardio-vascular surgery.
研制了一种新型的血钠、钾浓度连续测量系统。建立一个小的体外循环,血液通过Na和K敏感的玻璃电极。将该系统应用于清醒的大鼠和狗,证明了该方法用于生理分析的适用性。使用安装在电解质分析仪(SERA-230,Horiba,京都)温度控制单元上的Na和K敏感玻璃电极和参比电极进行[Na]和[K]的测量。在本系统中,用3.3mol/l KCl琼脂桥代替了商业分析仪中使用的液体接界,调节琼脂的pH和Na浓度至血液的pH和Na浓度。在生理范围内,测定Na的准确度约为1.5meq/l,K的准确度小于0.1meq/l。红细胞压积为42 ~ 51%的红细胞对阅读的影响在0.1 mV以内。对大鼠和狗进行了动物实验,并用于饮酒行为的分析。结果表明,该系统具有足够的灵敏度,可以检测到钠浓度的变化,随之而来的饮用行为,也可以检测到饮用水的盐度的差异。这些结果表明,本方法是一个潜在的工具,不仅为生理学研究,而且为临床应用领域的肾脏病和心血管手术。

项目成果

期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
日本生理学雑誌. 48-2-3. (1985)Japanese Journal of Physiology.
日本生理学杂志。48-2-3。(1985)日本生理学杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Eizo Sugimoto: "Changes in blood volume and blood sodium concentration during recovery from thermal dehydration." Japanese Journal of Biometeorology. 23(suppl.). 32 (1986)
Eizo Sugimoto:“热脱水恢复过程中血容量和血钠浓度的变化。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Hiroshi Nose: "Changes in blood volume and plasma sodium concentration after water intake in rats." American Journal of Physiology. 252. (1987)
Hiroshi Nose:“大鼠饮水后血容量和血浆钠浓度的变化。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Taketoshi Morimoto: Physiologist. 30. (1987)
森本武俊:生理学家。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
日本生理学雑誌. 48-2-3. (1985)
日本生理学杂志。48-2-3(1985)。
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  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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MORIMOTO Taketoshi其他文献

MORIMOTO Taketoshi的其他文献

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{{ truncateString('MORIMOTO Taketoshi', 18)}}的其他基金

Adaptability of older adults to heat with reference to water balance
老年人对热的适应性与水平衡的关系
  • 批准号:
    09470017
  • 财政年份:
    1997
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The role of water metabolism on the environmental adaptation
水代谢对环境适应的作用
  • 批准号:
    07307002
  • 财政年份:
    1995
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of a method for simultaneous measurement of blood flow and blood gases
血流和血气同时测量方法的开发
  • 批准号:
    07557191
  • 财政年份:
    1995
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of a system to measure circulating blood volume using infra-red laser.
开发使用红外激光测量循环血量的系统。
  • 批准号:
    04557008
  • 财政年份:
    1992
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
The relationship between blood volume, blood properties and work capacity.
血量、血液性质和工作能力之间的关系。
  • 批准号:
    04454145
  • 财政年份:
    1992
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Development of the continuous monitoring method of blood ammonia
血氨连续监测方法的研制
  • 批准号:
    63870010
  • 财政年份:
    1988
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
Studies on the interaction of regulatory mechanisms of body temperature, circulation and body fluid.
体温、循环、体液调节机制相互作用的研究。
  • 批准号:
    62440026
  • 财政年份:
    1987
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)

相似海外基金

Plasma Na natriuretic peptide is increased risk of carotid atherosclerosis in a Japanese urban population
血浆钠钠尿肽会增加日本城市人口颈动脉粥样硬化的风险
  • 批准号:
    26670320
  • 财政年份:
    2014
  • 资助金额:
    $ 2.69万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
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