Development of the method for continuous and in situ determination of ions in circulating blood.

研制了一种新型的血钠、钾浓度连续测量系统。建立一个小的体外循环，血液通过Na和K敏感的玻璃电极。将该系统应用于清醒的大鼠和狗，显示了该方法用于生理分析的适用性。使用Na和K敏感玻璃电极和安装在电解质分析仪（SERA-230，Horiba，京都）的温度控制单元上的参比电极进行[Na]和[K]的测量。在本系统中，用3.3mol/l KCl琼脂桥代替了商业分析仪中使用的液体接界，调节琼脂的pH和Na浓度至血液的pH和Na浓度。在生理范围内，测定Na的准确度约为1.5meq/l，K的准确度小于0.1meq/l。红细胞压积为42 ~ 51%的红细胞对阅读的影响在0.1 mV以内。对大鼠和狗进行了动物实验，并用于饮酒行为的分析。结果表明，该系统具有足够的灵敏度，可以检测到钠浓度的变化，随之而来的饮用行为，也可以检测到饮用水的盐度的差异。这些结果表明，本方法是一个潜在的工具，不仅为生理学研究，而且为临床应用领域的肾脏病和心血管手术。

A new continuous measuring system for blood Na and K concentrations was developed. A small extracorporeal circulation was made and the blood was passed through Na and K sensitive glass electrodes. Applying the system to conscious rats and dogs, applicability of the method for physiological analyses was shown.Measurements of [Na] and [K] were made using Na and K sensitive glass electrodes and a reference electrode mounted on a temperature control unit of an electrolyte analyzer (SERA-230, Horiba, Kyoto). In the present system, the liquid junction used in the commercial analyzer was replaced by 3.3 mol/1 KCl agar bridge adjusting pH and Na concentration of the agar to those of blood. The accuracy of the determination was about 1.5 meq/1 of Na and less than 0.1 meq/1 of K concentration within physiological range. The effect of erythrocytes with 42 to 51% of hematocrit on reading was within 0.1 mV.Animal experiments were performed on rats and dogs, and used for the analysis of drinking behavior. The results showed that the system is sensitive enough to detect the changes in Na concentration consequent to drinking behavior and also to detect difference of salinity of drinking water. These results indicate that the present method is a potential tool for not only for physiological studies but also for clinical use in the fields of nephrology and cardio-vascular surgery.