Development of a system to measure circulating blood volume using infra-red laser.

开发使用红外激光测量循环血量的系统。

• 批准号: 04557008
• 负责人: MORIMOTO Taketoshi
• 金额: $ 4.86万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557008/
• 关键词: infra-red laser; hematocrit; hemoglobin; blood volume; in vivo monitoring; oxygen delivery; 脱水; 循環虚脱; レーザー

Optical fiber probe implantable into vascular bed was developed for in situ determination of hematocrit continuously. This system consists of a flexible catheter-type optical fiber, a laser source, and a laser photo-detector. The catheter was constructed from double-lumen plastic tube(outer diameter, 1.5 mm ; inner diameter, 0.7 mm) in which transmitter and receiver plastic optical fibers were installed. At the top portion, a slit of 1 mm was made, and the hematocrit value of blood flowing through the slit was monitored continuously. As the light source, light with 805-nm wave length (an isosbestic wave length for oxy- and deoxy-hemoglobin) was obtained with diode (KLS-810-3W, Kette). Light intensity from the receiver fiber was monitored with a silicone photo diode (S2386-18K, Hamamatsu). The light intensity was amplified with a photosensor amplifier (C2719, Hamamatsu), and recorded with micro-computer (PC-386, EPSON) after A/D conversion.This system was evaluated in vitro, using suspension solution of washed erythrocyte with varied hemotocrit, which showed high coincidence with the theoretical value by Loewinger's formula. Animal experiments were performed on anesthetized rabbits. The fiber probe was inserted into the right atrium of rabbit. The hematocrit value was monitored continuously in vivo, and the value was compared the hematocrit value obtained with centrifugation method on sampled blood. Highly significant correlation was observed between the values obtained with conventional method and the present method, although about 1% of deviation was observed after infusion of saline. This finding suggests that the existence of uneven distribution of infused saline in blood vessel system.During the course of this experiment, we succeeded to develop a method to visualize oxygen distribution in tissue. By combining these two methods, it will be possible to analyze oxygen delivery in more detail.

研制了可植入血管床内的光纤探头，用于连续原位测定红细胞压积。该系统由柔性导管型光纤、激光光源和激光光电探测器组成。导管由双腔塑料管(外径1.5 mm，内径0.7 mm)构成，其中安装了发射器和接收器塑料光纤。在顶部切开1 mm狭缝，连续监测流过缝隙的血液的红细胞压积。以KLS-810-3W二极管为光源，获得波长为805 nm的光(氧合和脱氧血红蛋白的等色散波长)。用硅胶光电二极管(S2386-18K，滨松)监测来自接收器光纤的光强度。用光敏放大器(C2719，Hamamatsu)放大光强，经A/D转换后用微型机(PC-386，Epson)记录光强。用不同红细胞压积的洗涤红细胞悬浮液对该系统进行了体外评价，与Loewinger公式的理论值符合较高。动物实验在麻醉兔上进行。将纤维探头插入兔右心房。在活体内连续监测红细胞压积，并与离心法测得的血细胞比容进行比较。用常规方法测得的值与用本方法测得的值之间有极显著的相关性，尽管在注入生理盐水后有约1%的偏差。这一发现表明，血管系统中存在注入的生理盐水的不均匀分布。在本实验过程中，我们成功地开发了一种可视化组织中氧气分布的方法。通过结合这两种方法，将有可能更详细地分析氧气输送。

项目成果

森本武利他: "体液水分量の測定法とその問題点" 臨床透析. 9. 399-403 (1993)

Taketoshi Morimoto 等人：“测量体液水含量的方法及其问题”临床透析9. 399-403 (1993)。

K.Yaegashi et al.: "Progress of Microcirculation Reserch in Asia" Pergamon Press(in press), (1994)

K.Yeegashi 等：《亚洲微循环研究进展》Pergamon Press（正在出版），（1994 年）

K.Yaegashi, T.Itoh, T.Kousaka, T.Kinoshita, and T.Morimoto: "A new method to visualize tissue oxygen distribution using fluorescent microscopy." Jpn. J.Physiol.43. S97 (1993)

K.Yeegashi、T.Itoh、T.Kousaka、T.Kinoshita 和 T.Morimoto：“一种使用荧光显微镜可视化组织氧分布的新方法。”

T.Itoh et al.: "Progress of Microcirculation Reserch in Asia" Pergamon Press(in press), (1994)

T.Itoh 等：《亚洲微循环研究进展》Pergamon Press（正在出版），（1994 年）

T.Itoh et al.: "Progress of Microcirculation Reserch in Asia" Pergamon Perss(in press), (1994)

T.Itoh 等：《亚洲微循环研究进展》Pergamon Perss（出版中），（1994 年）