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Development of the continuous monitoring method of blood ammonia

血氨连续监测方法的研制

基本信息

批准号：
63870010
负责人：
MORIMOTO Taketoshi
金额：
$ 5.06万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

An attempt was made to develop a method to determine blood ammonia concentration continuously. As the sensor for ammonia detection, lr-Pd MOS diode was constructed, which showed a linear voltage change of 200 mV for 0-15 ppm change in ammonia. An another diode ammonia sensor (TGS # 814D by Figaro) was also tested which showed a lineality between 0-80 ppm. In addition, ammonia gas glass electrode which uses polytetrafluoro-ethylene as an ammonia permeable membrane showed wide range of lineality of electrode output, i.e. 0.1 -20 mM. Because the electrode system has advantages that it is usable for both gas and liquid and that modification of housing is easy, this system was used for further analyses.This system to measure ammonia concentration continuously was applied for the detection of blood urea, for which urease was immobilized to spherical cellulose gel with diameter of 50 m and packed in 70 1 of chamber. Blood was perfumed through the chamber and the increase of ammonia concentration was used for the detection of blood urea concentration, and the system gave highly significant correlation between the emf of the electrode and log of urea concentration between 0.1 and 100 mM. This system was successfully applied for the continuous monitoring of blood, urea concentration during hemodialysis of a patient was kidney disorder.These results suggests that the system developed in this study is applicable for the automated control of hemodialysis of patients with kidney disfunction and also for the monitoring of sanitary condition in confined space such as space craft.

尝试建立一种连续测定血氨浓度的方法。作为用于氨检测的传感器，构建了lr-Pd MOS二极管，其对于0-15 ppm的氨变化显示出200 mV的线性电压变化。还测试了另一个二极管氨传感器（Figaro的TGS#814D），其显示0-80 ppm之间的线性。此外，以聚乙烯为氨渗透膜的氨气玻璃电极显示出宽范围的电极输出线性，即0.1 - 20 mM。由于该电极系统具有气体和液体均可使用以及外壳易于修改的优点，将该系统用于进一步分析，并将该系统用于血尿素的检测，将尿素酶固定在直径为50 μ m的球形纤维素凝胶上，并装在70 μ l的小室中。将血液通过该室进行灌注，并利用氨浓度的增加来检测血液中的尿素浓度，并且该系统在0.1和100 mM之间给出了电极的电动势与尿素浓度的对数之间的高度显著的相关性。该系统成功地应用于血液的连续监测。结果表明，该系统可用于肾功能不全患者血液透析过程的自动化控制，也可用于肾功能不全患者血液透析过程中尿素浓度的监测。在密闭空间如宇宙飞船中的卫生条件。