An equipment for microdetermination of superoxide production of nentrophils.

用于微量测定中性粒细胞超氧化物产生的设备。

• 批准号: 60870034
• 负责人: NAKAMURA Hajime
• 金额: $ 0.83万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870034/
• 关键词: Superoxide; Neutrophils; PMA; スーパーオキサイド産生能; 好中球機能; 微量測定法

We have developped anequipment for mictrodetermination of superoxide production of polymorphonuclear neutrophils (PMNs).This equipment consists of special double beam spectrophotometer (550nm - 540nm) and data analysis system by microcomputer for eliminating a noise due to cells in the samples.PMNs were stimulated by either phorbol myristate acetate or Concanavalin A plus Cytocharasin D, Or FMLP, and their optimal Quantities were 0.01 to 5 ug for PMNs riched plasma, 0.1 to 5 ug for separated PMNs by PMA; 10 to 100 ug for Con A plus Cyt D, 0.1 to 1 umoles for FMLP. Only 5 to 10x10^4 PMNs were required for single/assay.By PMA, the superoxide production rates showed a good correlation between the value of plasma riched PMNs and the value of separated PMNs. By Con A plus Cyt D, The superoxide production rates showed a good correlation between the value of plasma riched PMNs and the values of separated PMNs in Newborns, however in adult, the values obtained from plasma riched PMNs were lower than those obtained from separated PMNs.This new device provided us to determine superoxide production rates of PMNs conveniently, and it is possible to determine it using plasma riched PMNs in newborn infants.

我们研制了一种用双光束分光光度计测定中性粒细胞超氧阴离子产生的装置用佛波酯、伴刀豆球蛋白A和细胞charasin D、或FMLP刺激PMNs，其最适浓度分别为：富含PMNs血浆0.01 ~ 5 μ g，PMA分离PMNs 0.1 ~ 5 μ g，Con A + Cyt D 10 ~ 100 μ g，FMLP 0.1 ~ 1 μ mol。单次测定仅需5 ~ 10 × 10 ^4个PMNs，PMA测定的血浆富集PMNs和分离PMNs的超氧化物生成速率具有良好的相关性。用Con A + Cyt D测定新生儿血浆富集的PMNs和分离的PMNs的超氧化物生成速率，两者之间有良好的相关性，而成人血浆富集的PMNs的超氧化物生成速率低于分离的PMNs的超氧化物生成速率，这一新装置为测定PMNs的超氧化物生成速率提供了方便。并且有可能使用新生儿中富含血浆的PMN来确定它。

项目成果

中村肇 他: 医学のあゆみ.

Hajime Nakamura 等人：医学史。

Hajime NAKAMURA: Biology of the Neonate. 52. 273-278 (1987)

Hajime NAKAMURA：新生儿生物学。

Hajime,NAKAMURA: "AN IMPROVED EQUIPMENT FOR MAASURING SUPEROXIDE PRODUCTION OF NEUTROPHILS." IGAKU_NO AYUMI. 140. 987-988 (1987)

Hajime,NAKAMURA：“用于测量中性粒细胞超氧化物产生的改进设备。”

中村 肇 他: 医学のあゆみ. 140. 987-988 (1987)

Hajime Nakamura 等人：医学史。140. 987-988 (1987)

Hiajime,NAKAMURA: "INHIBITORY ACTION OF BILIRUBIN ON SUPEROXIDE PRODUCTION BY POLYMORPHONUCLEAR LEUCOCYTES." BIOLOGY OF THE NEONATE. 52. 273-278 (1987)

Hiajime，NAKAMURA：“胆红素对多形核白细胞产生超氧化物的抑制作用。”