An equipment for microdetermination of superoxide production of nentrophils.

我们研制了一种用双光束分光光度计测定中性粒细胞超氧阴离子产生的装置用佛波酯、伴刀豆球蛋白A和细胞charasin D、或FMLP刺激PMNs，其最适浓度分别为：富含PMNs血浆0.01 ~ 5 μ g，PMA分离PMNs 0.1 ~ 5 μ g，Con A + Cyt D 10 ~ 100 μ g，FMLP 0.1 ~ 1 μ mol。单次测定仅需5 ~ 10 × 10 ^4个PMNs，PMA测定的血浆富集PMNs和分离PMNs的超氧化物生成速率具有良好的相关性。用Con A + Cyt D测定新生儿血浆富集的PMNs和分离的PMNs的超氧化物生成速率，两者之间有良好的相关性，而成人血浆富集的PMNs的超氧化物生成速率低于分离的PMNs的超氧化物生成速率，这一新装置为测定PMNs的超氧化物生成速率提供了方便。并且有可能使用新生儿中富含血浆的PMN来确定它。

We have developped anequipment for mictrodetermination of superoxide production of polymorphonuclear neutrophils (PMNs).This equipment consists of special double beam spectrophotometer (550nm - 540nm) and data analysis system by microcomputer for eliminating a noise due to cells in the samples.PMNs were stimulated by either phorbol myristate acetate or Concanavalin A plus Cytocharasin D, Or FMLP, and their optimal Quantities were 0.01 to 5 ug for PMNs riched plasma, 0.1 to 5 ug for separated PMNs by PMA; 10 to 100 ug for Con A plus Cyt D, 0.1 to 1 umoles for FMLP. Only 5 to 10x10^4 PMNs were required for single/assay.By PMA, the superoxide production rates showed a good correlation between the value of plasma riched PMNs and the value of separated PMNs. By Con A plus Cyt D, The superoxide production rates showed a good correlation between the value of plasma riched PMNs and the values of separated PMNs in Newborns, however in adult, the values obtained from plasma riched PMNs were lower than those obtained from separated PMNs.This new device provided us to determine superoxide production rates of PMNs conveniently, and it is possible to determine it using plasma riched PMNs in newborn infants.