An equipment for microdetermination of superoxide production of nentrophils.

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We have developped anequipment for mictrodetermination of superoxide production of polymorphonuclear neutrophils (PMNs).This equipment consists of special double beam spectrophotometer (550nm - 540nm) and data analysis system by microcomputer for eliminating a noise due to cells in the samples.PMNs were stimulated by either phorbol myristate acetate or Concanavalin A plus Cytocharasin D, Or FMLP, and their optimal Quantities were 0.01 to 5 ug for PMNs riched plasma, 0.1 to 5 ug for separated PMNs by PMA; 10 to 100 ug for Con A plus Cyt D, 0.1 to 1 umoles for FMLP. Only 5 to 10x10^4 PMNs were required for single/assay.By PMA, the superoxide production rates showed a good correlation between the value of plasma riched PMNs and the value of separated PMNs. By Con A plus Cyt D, The superoxide production rates showed a good correlation between the value of plasma riched PMNs and the values of separated PMNs in Newborns, however in adult, the values obtained from plasma riched PMNs were lower than those obtained from separated PMNs.This new device provided us to determine superoxide production rates of PMNs conveniently, and it is possible to determine it using plasma riched PMNs in newborn infants.