ANALYSIS OF MOLECULAR MECHANISMS WHEREBY THIOREDOXIN TRASNGENIC MICE ARE RESISTANT TO INFLAMMATION AND STRESS

硫氧还蛋白转基因小鼠抗炎症和应激的分子机制分析

基本信息

  • 批准号:
    14370074
  • 负责人:
  • 金额:
    $ 9.54万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

THIOREDOXIN (TRX) IS A SMALL PROTEIN WITH A REDOX ACTIVE DITHIOL/DISULFIDE IN ITS ACTIVE SITE SEQUENCE: -CYS-GLY PRO-CYS-. TRX IS INDUCED BY A VARIETY OF OXIDATIVE STRESS AND SECRETED FROM CELLS. TRX-TRANSGENIC (TRX TG) MICE ARE MORE RESISTANT TO STRESSES INDUCED BY ISCHEMIA-REPERFUSION AND ULTRAVIOLET IRRADATION AND SURVIVE LONGER THAN WILD TYPE MICE. BLEOMYCIN-INDUCED LEUKOCYTE INFILTRATION INTO THE, LUNG INTERSTITIAL SPACE WAS DRAMTICALLY SUPPRESSED IN TRX TG MICE. ACUTE HEPATIC INJURY CAUSED BY THIOACETAMIDE WAS ALSO SUPPRESSED IN TRX TG MICE. MOREOVER, ADMINISTRATION OF RECOMBINANT THIOREDOXIN PROTEIN ALSO SUPPRESSED THE EXTRAVASATION OF LEUKOCYTES, THE INTRACELLULAR GENERATION OF REACTIVE OXYGEN SPECIES AND CELLULAR APOPTOSIS. THESE RESULTS SUGGEST THE THERAPEUTIC POTENTIAL OF RECOMBINANT THIOREDOXIN FOR STRESS-ASSOCIATED DISORDERS.
硫氧还蛋白(TRX)是一种小分子蛋白质,在其活性位点序列中具有氧化还原活性二硫醇/二硫化物:-Cys-Gly pro-Cys-。TRX由多种氧化应激诱导并从细胞中分泌。TRX转基因小鼠(TRX TG)对缺血再灌注和紫外线照射诱导的应激具有更强的抵抗力,并且比野生型小鼠存活更长时间。博莱霉素诱导的白细胞向TRX TG小鼠肺间质空间的浸润受到显著抑制。在TRX TG小鼠中,硫代乙酰胺引起的急性肝损伤也受到抑制。此外,重组硫氧还蛋白还能抑制白细胞的抽提、细胞内活性氧的产生和细胞凋亡。这些结果表明重组硫氧还蛋白对应激相关疾病的治疗潜力。

项目成果

期刊论文数量(48)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
I.Hattori, et al.(10名, 3番): "Intravenous administration of thioredoxin decreases brain damage following transient focal cerebral ischemia in mice."Antioxidants and Redox Signaling. 6. 81-87 (2004)
I. Hattori 等人(10 人,第 3 期):“静脉注射硫氧还蛋白可减少小鼠短暂局灶性脑缺血后的脑损伤。”抗氧化剂和氧化还原信号传导。
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    0
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  • 通讯作者:
H.Okuyama, et al.: "Overexpression of thioredoxin prevents acute hepatitis caused by thioacetamide or lipopolysaccharide in mice"Hepatology. 37. 1015-1025 (2003)
H.Okuyama 等人:“硫氧还蛋白的过度表达可预防小鼠由硫代乙酰胺或脂多糖引起的急性肝炎”肝病学。
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    0
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  • 通讯作者:
H.Nakamura, et al.: "Enhanced resistancy of thioredoxin-transgenic mice against influenza virus-induced penumonia"Immunology Letters. 82. 165-170 (2002)
H.Nakamura等人:“硫氧还蛋白转基因小鼠对流感病毒诱导的肺炎的增强抵抗力”免疫学快报。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
H.Okuyama, et al.(7名, 2番): "Overexpression of thioredoxin preventgs acute hepatitis caused by thioacetamide or lipopolysaccharide in mice"Hepatology. 37. 1015-1025 (2003)
H. Okuyama 等人(7 人,第 2 期):“硫氧还蛋白的过度表达可预防小鼠硫代乙酰胺或脂多糖引起的急性肝炎”《肝病学》。
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  • 影响因子:
    0
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  • 通讯作者:
K.Shioji, et al.: "Overexpression of thioredoxin-1 in transgenic mice attenuates adriamycin-induced cardiotoxicity"Circulation. 106. 1403-1409 (2002)
K.Shioji 等人:“转基因小鼠中硫氧还蛋白-1 的过度表达可减弱阿霉素诱导的心脏毒性”循环。
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    0
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NAKAMURA Hajime其他文献

NAKAMURA Hajime的其他文献

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{{ truncateString('NAKAMURA Hajime', 18)}}的其他基金

Relationship between cortical spreading depression (CSD) and high mobility group box 1 (HMGB1) as synergistic deteriorating factors in cerebral ischemia.
皮质扩散抑制 (CSD) 和高迁移率族蛋白 1 (HMGB1) 作为脑缺血协同恶化因素之间的关系。
  • 批准号:
    15K10301
  • 财政年份:
    2015
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of new method detecting redox regulation against oxidative stress
开发检测氧化应激氧化还原调节的新方法
  • 批准号:
    23390152
  • 财政年份:
    2011
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a novel method to measure unsteady heat transfer caused by flow turbulence using infrared thermography
开发一种利用红外热成像测量湍流引起的不稳定传热的新方法
  • 批准号:
    22560214
  • 财政年份:
    2010
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Imaging of regional cerebral blood flow in the surrounding of cerebral infarction using laser speckle flowmetry system
激光散斑血流仪对脑梗死周围局部脑血流成像
  • 批准号:
    21791357
  • 财政年份:
    2009
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Research on brain hypothermia for neonatal hypoxic-ischemic brain damage
脑低温治疗新生儿缺氧缺血性脑损伤的研究
  • 批准号:
    12470215
  • 财政年份:
    2000
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of molecular mechanisms on cytoprotective effect of thioredoxin for host defense and aging prevention
硫氧还蛋白对宿主防御和衰老预防的细胞保护作用的分子机制分析
  • 批准号:
    12670113
  • 财政年份:
    2000
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular genetic study on G6PD deficiency in Philippines
菲律宾 G6PD 缺乏症的分子遗传学研究
  • 批准号:
    11691210
  • 财政年份:
    1999
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Study on the characteristic plasticity and preventive mechanisms against neonatal brain damage.
新生儿脑损伤特征可塑性及预防机制研究
  • 批准号:
    09470241
  • 财政年份:
    1997
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of Rere-Earth Iron based hard magnetic thin films with high energy products
高能积稀土铁基硬磁薄膜的研制
  • 批准号:
    07555652
  • 财政年份:
    1995
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the effects of nitric oxide on cerebral blood flow in developmental brain.
一氧化氮对发育期大脑脑血流影响的研究
  • 批准号:
    07457244
  • 财政年份:
    1995
  • 资助金额:
    $ 9.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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暴露于大麻的 HIV Tat 转基因小鼠食欲学习过程中的体内钙成像
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使用 EP400 基因转基因小鼠阐明精神分裂症/自闭症谱系障碍的病理生理学
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