Establishment of animal models with acuto myocardial infaroction and/or suuden death

1. 选取4 ~ 6月龄的冠心病痉挛迷你猪，饲喂含胆固醇（2%）和胆酸的合成饲粮6个月，建立急性心肌梗死和/或猝死动物模型。在胆固醇喂养第1个月时，采用冠状动脉导管对最小的冠状动脉进行球囊去内皮化。剥落部位在剥落3个月和4个月选择性x射线照射两次，每次剂量为1500 rad。最后一次x射线照射1个月后，血清素ic引起冠状动脉痉挛，冠状动脉造影和心电图- st改变记录冠状动脉痉挛与心肌缺血的演变。在严重的冠状动脉痉挛引起的病例中，偶尔会出现猝死。在持续严重痉挛超过30分钟的猪中，由于新形成的毛细血管破裂引起的壁内出血出现在痉挛部位增厚的内膜中。组织学上管腔沿出血方向狭窄，痉挛部位为56(+2)7%，明显大于单次注射5 -羟色胺导致猪暂时性缺血的痉挛部位。因此，与冠状动脉痉挛密切相关的壁内出血和晚期管腔狭窄事件可能为建立心肌梗死和/或死亡研究的有用动物模型提供线索。急性心肌梗死伴高脂血症动物模型Watanabe Hetirable Hyperlipidemie兔（WHHL）是一种持续遗传高脂血症系的日本白兔。为了培育具有诱发性心肌梗死特征的染色体，按时间顺序观察冠状动脉粥样硬化的进展和对心脏血管舒张剂的反应。10个月后，冠状动脉造影显示右侧冠状动脉明显狭窄。缺血性ECG-ST改变缺血的家兔已近交系获得一种快速心肌梗死品系。少

1. Animal model of acute myocardial infarction and/or sudden death relating to the evolution of cornary spasmcottingen miniature pigs 4-6 months of age were fed synthetic diet containing cholesterol (2%) and cholic acid for 6 months. Balloondeendothelialization of the lest cotonary artery was performed using coronary cathetreization at 1st month of cholesterol feeding. Denuded site was selectively X-irradiated twice with a dose of 1500 rad each at 3 and 4 months of denudation. One month after the last X-irradiation, coronary spasm was provoked by serotonin ic and the evolution of coronary spasm associted with myocardial ischemia was documented by coronary antiography and ECG-ST change. Suffen death was occasionally noted in cases with severe coronary spasm induced. In pigs with persistent severe spasm over 30 minutes, intramural hemorrhage due to rupture of newly developed capillaries was moted in the thickened intima of the spastic site. Histological luminal narrowing along the hemorr … More gagic site was 56(+2)7%, which was significantly larger than that of the spasticsite in pigs rendered ischmic transiently by a single bolus injection of serotonin. Thus, the events of both intramural hemorrhage and advanced luminal stenosis, intimately related to coronary artery spasm may providce a clue to develop a useful animal modedl for the study of myocardial infaction and/or suffed death.2. Nimal model of acute myocadial infarction with hyperlipidemiaThe WHHL (Watanabe Hetirable Hyperlipidemie) rabbit is a strain of Japanese white rabbit with a consistrntly inherited hyperlipidemic train. To breed a stain with a trait of provokable myocardial infaction, progression of coronary atherosclerosis and responeses to vasotonic agents of the heart were ezmained chronologigally. Afte 10 months of age, siginificant coronary stenosis was frequently noted angiographically along the right coronary artery. Rabbits with ischemic ECG-ST change ischemia have been inbred to obtain a strain with soontaneous myocardial infaction. Less