Establishment of animal models with acuto myocardial infaroction and/or suuden death

急性心肌梗死和/或猝死动物模型的建立

• 批准号: 61870037
• 负责人: NAKAMURA Motoomi
• 金额: $ 17.41万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61870037/
• 关键词: Coronary artery spasm; Myocardial infarction; Intramural hemorrhage; Serotonin; Miniature Pig; WHHL rabbvit; Hyperlipifemia; WHHL; 急性心筋梗塞; 冠動脈攣縮; 狭心症; 動物モデル

1. Animal model of acute myocardial infarction and/or sudden death relating to the evolution of cornary spasmcottingen miniature pigs 4-6 months of age were fed synthetic diet containing cholesterol (2%) and cholic acid for 6 months. Balloondeendothelialization of the lest cotonary artery was performed using coronary cathetreization at 1st month of cholesterol feeding. Denuded site was selectively X-irradiated twice with a dose of 1500 rad each at 3 and 4 months of denudation. One month after the last X-irradiation, coronary spasm was provoked by serotonin ic and the evolution of coronary spasm associted with myocardial ischemia was documented by coronary antiography and ECG-ST change. Suffen death was occasionally noted in cases with severe coronary spasm induced. In pigs with persistent severe spasm over 30 minutes, intramural hemorrhage due to rupture of newly developed capillaries was moted in the thickened intima of the spastic site. Histological luminal narrowing along the hemorr … More gagic site was 56(+2)7%, which was significantly larger than that of the spasticsite in pigs rendered ischmic transiently by a single bolus injection of serotonin. Thus, the events of both intramural hemorrhage and advanced luminal stenosis, intimately related to coronary artery spasm may providce a clue to develop a useful animal modedl for the study of myocardial infaction and/or suffed death.2. Nimal model of acute myocadial infarction with hyperlipidemiaThe WHHL (Watanabe Hetirable Hyperlipidemie) rabbit is a strain of Japanese white rabbit with a consistrntly inherited hyperlipidemic train. To breed a stain with a trait of provokable myocardial infaction, progression of coronary atherosclerosis and responeses to vasotonic agents of the heart were ezmained chronologigally. Afte 10 months of age, siginificant coronary stenosis was frequently noted angiographically along the right coronary artery. Rabbits with ischemic ECG-ST change ischemia have been inbred to obtain a strain with soontaneous myocardial infaction. Less

1.用4-6月龄小型猪制作急性心肌梗塞和/或猝死动物模型，饲喂含2%胆固醇和胆酸的人工饲料6个月。在胆固醇喂养的第1个月，使用冠状动脉导管术对最后的冠状动脉进行血管内皮化。剥蚀部位选择性X射线照射两次，每次剂量为1500拉德，在3个月和4个月的剥蚀。末次X线照射后1个月，用5-羟色胺诱发冠状动脉痉挛，通过冠状动脉造影和心电图ST段改变记录冠状动脉痉挛的演变过程。在诱发严重冠状动脉痉挛的病例中偶见窒息死亡。在持续严重痉挛超过30分钟的猪中，在痉挛部位增厚的内膜中发现由于新生毛细血管破裂引起的壁内出血。组织学管腔狭窄沿着出血 ...更多信息 gagic部位为56 ± 2.7%，显著大于通过单次推注5-羟色胺使猪短暂缺血的痉挛部位。因此，与冠状动脉痉挛密切相关的壁内出血和晚期管腔狭窄事件可能为开发用于研究心肌梗死和/或死亡的有用动物模型提供线索。日本白色家兔WHHL（Watanabe Hetirable Hypertensive）是一种遗传性高血压病的日本品系。为了培育具有可诱发心肌梗死特征的染色剂，按时间顺序记录冠状动脉粥样硬化的进展和对心脏血管紧张剂的反应。10月龄后，血管造影经常发现右冠状动脉沿着有明显的冠状动脉狭窄。用缺血性心电图-ST改变的家兔进行近交系，获得了一个自发性心肌梗死的品系。少

项目成果

Nagasawa K. et al.: Circulation Part II. 76. 375 (1987)

Nagasawa K. 等人：循环第二部分。

Yamamoto Y. et al.: Circulation Research. 61. 772-778 (1987)

Yamamoto Y. 等人：循环研究。

Yamamto Y.: "Pathogenesis of coronaty artesy spasm in miniature swine with regional intimal thickening after balloon decudation." Circulation Reserarh. 60. 113-121 (1987)

Yamamto Y.：“小型猪冠状动脉痉挛的发病机制，球囊脱落后局部内膜增厚。”

Maruoka Y: Br J Exp Pathol. 67. 33-42 (1986)

Maruoka Y：Br J Exp Pathol。

Ohzono K.: Circ Res. 59(1). 63-73 (1986)

Ohzono K.：Circ Res。