DEVELOPMENT OF BIOSENSORS FOR DETERMINING ENZYME ACTIVITIES

开发用于测定酶活性的生物传感器

• 批准号: 63860026
• 负责人: NAGAYAMA Fumio
• 金额: $ 3.97万
• 依托单位: TOKYO UNIVERSITY OF FISHERIES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63860026/
• 关键词: Biosensor; Enzyme activity; GOT; GPT; LDH; Serum; 酵素; 酵素計測; バイオセンサー

Several biosensor systems for determining enzyme activities have been developed. 1. Lactate dehydrogenase (lactate + NAD - pyruvate + NADH) was determined with the oxygen electrode on which immobilized pyruvate oxidase (pyruvate + O_2 + H_20 -> acetyl phosphate + CO_2 + H_2O_2)membrane was equipped. 2. Activity of glutamate-pyruvate transaminase(GPT; alanine + oxoglutarate -> pyruvate + glutamate) was determined with the same electrode. 3. A dual enzyme sensor system was devised for simultaneous determination of lactate dehydrogenase and glutamate-pyruvate transaminase. 4. Activity of glutamate-oxaloacetate transaminase (GOT; aspartate + oxoglutarate -> oxaloacetate + glutamate) was determined with the oxygen electrode on which immobilized oxaloacetate decarboxylase (oxaloacetate -> pyruvate + CO_2) and pyruvate oxidase membrane was equipped. T hese sensor systems may be applicable for diagnostic determination of enzymes in fish sera.

已经开发了几种用于测定酶活性的生物传感器系统。1. 采用固定化丙酮酸氧化酶（丙酮酸+ O_2 + H_20 ->乙酰磷酸+ CO_2 + H_2O_2）膜，在氧电极上测定乳酸脱氢酶（乳酸+ NAD -丙酮酸+ NADH）。2. 用同一电极测定谷氨酸-丙酮酸转氨酶（GPT；丙氨酸+氧戊二酸->丙酮酸+谷氨酸）的活性。3. 设计了一种双酶传感器系统，用于乳酸脱氢酶和谷丙转氨酶的同时测定。4. 采用固定化草酰乙酸脱羧酶（草酰乙酸->丙酮酸+ CO_2）和丙酮酸氧化酶膜在氧电极上测定了谷草酰乙酸转氨酶（GOT；天冬氨酸+氧酰乙酸->草酰乙酸+谷氨酸）的活性。这些传感器系统可用于鱼血清中酶的诊断测定。

项目成果

H.Okuma: "Biosensor system for continuous flow determination of enzyme activities. I. Determination of glucose oxidase and lactic dehydrogenase activities." Enzyme and Microbial Technology Vol. 11, p.824-829, 1989.

H.Okuma：“用于连续流动测定酶活性的生物传感器系统。I.葡萄糖氧化酶和乳酸脱氢酶活性的测定。”

Hirokazu Okuma: "Biosensor system for continuous flow determination of enzyme activities.I.Determination of glucose oxidase and lactic dehydrogenase activities." Enzyme and Microbial Technology. 11. 824-829 (1989)

Hirokazu Okuma：“用于连续流动测定酶活性的生物传感器系统。I.葡萄糖氧化酶和乳酸脱氢酶活性的测定。”

Hirokazu Okuma: "Biosensor system for continuous flow determination of enzyme activities.I.Determination of glucose oxidase and lactic dehydrogenase activities" Enzyme Microb.Technol.,. 11. 824-829 (1989)

Hirokazu Okuma：“用于连续流动测定酶活性的生物传感器系统。I.葡萄糖氧化酶和乳酸脱氢酶活性的测定”Enzyme Microb.Technol.，。